Exam 3 key problems Flashcards
Who does the blood evidence found at the crime scene most likely belong to?
a) the victim
b) suspect 1
c) suspect 2
d) both the victim and suspect 1
e) both the victim and suspect 2
a) the victim
Who does the hair evidence found at the crime scene most likely belong to?
a) the victim
b) suspect 1
c) suspect 2
d) both the victim and suspect 1
e) both the victim and suspect 2
b) suspect 1
What does the forensic DNA analysis say most clearly about suspect 1?
a) She is innocent
b) she is guilty
c) She was at the scene at the time of the crime
d) someone matching her genetic fingerprint was at the crime scene
e) there is no direct DNA evidence that she was at the crime scene
b) she is guilty
What does the forensic DNA analysis say most clearly about suspect 2?
a) he is innocent
b) he is guilty
c) He was at the scene at the time of the crime
d) someone matching his genetic fingerprint was at thw crime scene
e) there is no direct DNA evidence that he was at the crime scene
a) he is innocent
and
e) there is no direct DNA evidence that she was at the crime scene
The use or alteration of cells or biochemicals to provide a useful product descibes
a) recombinant DNA technology
b) transgenic technology
c) biotechnology
d) gene targeting
c) biotechnology
Which of the technologies listed below is a valuable method for mass-producing drugs and other useful proteins?
a) recombinant DNA technology
b) transgenic technology
c) biotechnology
d) gene targeting
a) recombinant DNA technology
Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location
a) restriction enzyme
b) plasmid
c) bacteriophage
d) vector
a) restriction enzyme
Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transfering the circle of DNA to cells of another species, Which of the below describe the circular piece of DNA from a bacterial cell?
a) restriction enzyme
b) plasmid
c) bacteriophage
d) vector
b) plasmid
Which gene transfer technique involves a tiny needle, which is used to inject DNA into a cell lacking that DNA sequence?
a) electroporation
b) liposome transfer
c) microinjection
d) particle bombardment
c) microinjection
The process of ______ involves the introduction of a gene into a cell where it exchanges places with its counterpart in the host cell.
a) transgenic technology
b) gene targeting
c) knockout technology
d) recombinant DNA technology
b) gene targeting
Genetic enginerring manipulates gene products at the level of the
a) protein
b) amino acid
c) DNA
d) RNA
c) DNA
A cDNA version of a gene includes
a) codons for a mature mRNA
b) sequences corresponding to promoters
c) sequences corresponding to introns
a) codons for mature mRNA
________ are used to select genes of interest from a genomic library
a) Restriction enzymes
b) cloning vectors
c) DNA probes
d) gene targets
c) DNA probes
a) Draw the restriction map
b) The mixture of fragments produced by the combined enzymes is cleaved with the enzyme EcoRI, resulting in the loss of the 3-kb fragment ( band stained with ethidium bromide on an agarose gel) and the appearance of a band stained with ethidium bromide representing a 1.5-kb fragment. Mark the EcoRI cleavage site on the restriction map
a) Design the sense and antisense primer including the restriction enzyme need it to clone in the vector pREST A. Indicate the 5’ position
Sense: 5’ BamHI or Kpn I or Eco RI - TGGCCGTGTG
Antisense: 5’ Hind III - CCTAAAGTTCCT
b) Calculate the Tm of the oligonucleotieds
Tm sense= 2(3) + 4(7) = 6 + 28 = 34C
Tm antisense= 2(7) + 4(5) = 14 + 20 = 34C
C) Indicate the PCR steps to amplify this gene including the temperature and the time for each one
In our experiment, the normal hepatocyte sample is labeled with fluoresces red, and the sample with cncerous epatocytes were labeled with fluoresces blue. We apply the sample to the chip and then scan the chip. How many genes in chip depicted bellow are expressed only in cancer cell?
a) 15
b) 11
c) 8
d) 4
b) 11
How many genes in chip depicted bellow is expressed in cancer and normal cells?
a) 15
b) 11
c) 8
d) 4
d) 4
True or False: Sequence similarity searching algorithms like BLAST are based on the premise that if two sequences are similar then they are likely to be homologous
True
True or False: A motif is a distinctive pattern of amino acids, conserved across many proteins, which gives a particular fuction to the protein
True
Expression libraries are made with
a) Complementary DNA (cDNA)
b) Ribosomal RNA (rRNA)
c) messanger RNA (mRNA)
d) Double stranded DNA (dsDNA)
a) Complementary DNA (cDNA)
To understand the functions of genes we use reverse genetics and some of its tools are
a) RNAi
b) Knockout study
c) Microarray
d) a and b
e) a, b, and c
e) A, B, and C
Which of the following tools of recombinant DNA technology is INCORRECTLY paired with its use?
a) REstriction endonuclease - production of DNA fragments for gene cloning
b) DNA ligase - enzyme that cuts DNA, creating sticky ends
c) DNA polymerase - copies DNA sequences in the polymerase chain reaction
d) Reverse transcriptase - production of cDNA from mRNA
b) DNA ligase - enzyme that cuts DNA, creating sticky ends
The “Southern” technique involves:
a) The detection of RNA fragments on membranes by specific radioactive antibodies
b) The detection of DNA fragments on membranes by a radioactive DNA probe
c) The detection of proteins on membranes using a radioactive DNA probe
d) The detection of proteins on membranes using specific radioactive antibodies
e) The detection of DNA fragments on membranes by specific radioactive antibodies
b) The detection of DNA fragments on membranes by a radioactive DNA probe
“Gene library” is a term used to describe:
a) A computerized listing of known DNA sequences.
b) Bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal
c) A collection of books about recombinant DNA technology
d) Particle bombardment
b) Bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal
Extra info: A method for transferring protein from an electrophoresis gel to a permanent membrane or filter.
Western Blotting
Extra Info: A method for transferring mRNA from an electrophoresis gel to a permanent membrane or filter.
Northern Blotting
A multicellular organism that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is
a) transversion
b) transition
c) transgenic
d) transformant
c) transgenic
The first drug produced using recombinant DNA tecnology is used to treat
a) hemophilia
b) dwarfism
c) heart attack
d) diabetes
d) diabetes
True or False: Restriction enzymes hydrolyze the phosphodiester backbone once on one strand
True
Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
a) electroporation
b) liposome transfer
c) microinjection
b) liposome transfer
The “ northern blot” technique involves:
a) the detection of RNA fragments on membranes by specific radioactive antibodies
b) the detection of DNA fragments on membranes by a radioactive DNA probe
c) the detection of proteins on membranes using a radioactive DNA probe
d) the detection of proteins on membranes using specific radioactive antibodies
e) the detection of DNA fragments on membranes by specific radioactive antibodies
a) the detection of RNA fragments on membranes by sepcific radioactive antibodies
True or False: Functional genomics seeks to understand the function of genes and how they determine phenotypes
True
