Exam 3 Flashcards
In which stage of the cell cycle are sister chromatids separated and pulled to the opposite poles of the cell?
Anaphase
What relieves mechanical strain that builds up (twisting forces) during replication in E. coli?
topoisomerase
Strand initiation in DNA replication is accomplished when an enzyme lays down a primer. Of what molecule is that primer composed?
RNA
A group of cells is assayed for DNA content immediately following mitosis and are found to have an average of 8 picograms of DNA per nucleus. Those cells would have _____ picograms of DNA at the end of the S phase and _____ picograms of DNA at the end of G2.
16, 16
Why is DNA replication called semiconservative?
Each daughter duplex contains one strand from the parent structure.
In human and many other eukaryotic species’ cells, the nuclear membrane has to disappear to permit
the attachment of microtubules to kinetochores
The specific site on the bacterial chromosome at which replication begins is called the ________
origin
Replication moves outward from the origin in ________ direction(s) and is said to be ________
both, bidirectional
What proteins bind selectively to single-stranded DNA and are responsible for keeping it extended and preventing it from being rewound and becoming double stranded?
single-stranded DNA binding (SSB) proteins
What type of hypothesized replication (Meselson-Stahl model) results in one duplex containing both parental strands and the other duplex containing two new strands of DNA after the two parental strands had served as templates for the two daughter strands?
conservative replication
In which direction are nucleotides laid down in the new strand while DNA is being synthesized?
5’—>3‘
The RNA primers that initiate replication are subsequently _____ and the resulting gap in the strand is _______ DNA and then sealed by _______
removed, filled in with, DNA ligase
In which direction do the DNA strands grow during replication?
one toward the replication fork and one away from the replication fork
New strand initiation during replication is carried out by an enzyme that makes a short RNA molecule that is used as a primer; the enzyme is a distinct type of RNA polymerase, called _______
Primase
Cells that are in a non-dividing state are in which phase?
G0
What are two copies of the same DNA (have the same exact sequence)?
Sister Chromatids
What are two copies of the same genes, but not always the same sequence (one from mom, one from dad)?
Homologous Chromosomes
In which stage of the cell cycle are sister chromatids separated and pulled to the opposite poles of the cell?
Anaphase
Meiosis is the separation of:
Both Sister Chromatids and Homologous Chromosomes
During replication, DNA is constructed in small segments called ________ that are rapidly linked to longer pieces of DNA synthesized earlier.
Okazaki fragments
A group of cells is assayed for DNA content immediately following mitosis and are found to have an average of 8 picograms of DNA per nucleus. Those cells would have _____ picograms of DNA at the end of the S phase and _____ picograms of DNA at the end of G2.
16, 16
The enzyme that joins the small fragments of the lagging strand together into a continuous strand is called
DNA ligase
Proteins that are involved in the regulation of the cell cycle, and that show fluctuations in concentration during the cell cycle, are called:
Cyclins
If a liver cell of an animal has 24 chromosomes total, the sperm cells from this animal will have how many chromosomes?
12
DNA is undamaged, therefore DNA will be allowed to replicate.
G1
Chromosomes have replicated correctly and MPF is present.
G2
Spindle fibers are properly attached to sister chromatids at the kinetochore
M
Where does Glycolosis take place in eukaryotic cells?
Cytoplasm (Cytosol)
What types of molecules are the major structural components of the cell membrane?
Phospholipids and Proteins
Which eukaryotic organelle or organelles resemble prokaryotic cells?
Mitochondria
Chloroplasts
A series of enzymes catalyze the reaction X to Y to Z. Product A binds to the enzyme that converts X to Y at a position remote from its activation site. The binding decreases the activity of the enzyme. What is substance A?
An allosteric inhibitor
What occurs in meiosis but not in mitosis?
synapsis of chromosomes
What is a major difference between mitosis and meiosis I in a diploid organism?
Sister chromatids separate in mitosis; homologous pairs of chromosomes separate in meiosis I
Whether during mitosis or meiosis, sister chromatids are held together by proteins referred to as cohesins. Such molecules must ________.
be removed before sister chromatids can separate
Sister chromatids separate from each other during ________.
mitosis and meiosis II
Why might adding inorganic phosphorus to a reaction mixture where glycolysis is rapidly proceeding help sustain the metabolic pathway?
The metabolic intermediates of glycolysis are phosphorylated.
In humans, xeroderma pigmentosum (XP) is a disorder of the nucleotide excision repair mechanism. These individuals are unable to repair DNA damage caused by ultraviolet light. Which of the following are the most prominent types of DNA lesions in individuals suffering from xeroderma pigmentosum?
thymine dimers
What is the role of DNA ligase in the elongation of the lagging strand during DNA replication?
It joins Okazaki fragments together.
Researchers found E. coli that had mutation rates 100 times higher than normal. Which of the following is the most likely cause of these results?
The proofreading mechanism of DNA polymerase was not working properly.
Which of the following reactions is the correct description of the beginning of the Citric Acid (Kreb’s) Cycle?
Acetyl CoA + oxaloacetate -> citrate
What are the products of glycolysis?
2 pyruvate
2 (net) ATP
2 NADH
The product of the p53 gene ________.
inhibits the cell cycle
What does NOT occur during mitosis?
replication of the DNA
The M-phase checkpoint ensures that all chromosomes are attached to the mitotic spindle. If this does NOT happen, cells would most likely be arrested in ________.
metaphase
FtsZ is a bacterial cytoskeletal protein that forms a contractile ring involved in bacterial cytokinesis. Its function is analogous to ________.
the cleavage furrow of eukaryotic animal cells
Cyclin-dependent kinase (Cdk) is ________.
an enzyme that attaches phosphate groups to other proteins
You are observing diseased cells in a medical laboratory. Upon close inspection, you notice that the cells contain many food vacuoles, yet the cells are dying of starvation. Which organelle is most likely to be the one malfunctioning within these cells?
Lysosomes
Where are the proteins of the electron transport chain located?
Mitochondrial inner membrane
The final electron acceptor of the electron transport chain that functions in aerobic oxidative phosphorylation is ________.
oxygen
What type of bonds form between the monomers that make up carbohydrates?
Glycosidic
Carbon skeletons to be broken down during cellular respiration can be obtained from:
Polysaccharides, Lipids, and Proteins
What is diffusion?
It is a passive process in which molecules move from a region of higher concentration to a region of lower concentration.
Enzymes increase the speed of certain biological chemical reactions by
bringing reactants into physical contact with one another
What type of bonds hold the nucleotides together to make up the backbone of DNA?
Covalent
Muscle cells in oxygen deprivation convert pyruvate to ______ and in this step gain ______.
Lactic acid, NAD+
For a cell to function at an optimum, the ideal ratio between surface area and volume is?
Low volume and high surface area
Which of the following would you expect of a eukaryote lacking telomerase?
a reduction in chromosome length in gametes
Eukaryotic telomeres replicate differently than the rest of the chromosome. This is a consequence of what?
gaps left at the 5’ end of the lagging strand
Under some conditions, too much pyruvate is produced and it is more energy favorable for the cell to slow down glycolysis. What would be most likely to act as a feedback inhibitor for the process of glycolysis?
**
What is a major difference between meiosis II and mitosis in a diploid animal?
Meiosis II occurs in a haploid cell, and mitosis occurs in diploid cells.
What is a major difference between mitosis and meiosis I in a diploid organism?
Sister chromatids separate in mitosis; homologous pairs of chromosomes separate in meiosis I.
Which of the following occurs in meiosis but not in mitosis?
synapsis of chromosomes
Homologous chromosomes ________.
carry information for the same traits
Whether during mitosis or meiosis, sister chromatids are held together by proteins referred to as cohesins. Such molecules must ________.
be removed before sister chromatids can separate
Vinblastine is a standard chemotherapeutic drug used to treat cancer. Because it interferes with the assembly of microtubules, its effectiveness must be related to ________.
disruption of mitotic spindle formation
The M-phase checkpoint ensures that all chromosomes are attached to the mitotic spindle. If this does NOT happen, cells would most likely be arrested in ________.
metaphase
If a cell has accumulated DNA damage, it is unlikely to ________.
pass the G2 checkpoint
How is plant cell cytokinesis different from animal cell cytokinesis?
Plant cells deposit vesicles containing cell-wall building blocks on the metaphase plate; animal cells form a cleavage furrow.
What sequence correctly represents the flow of electrons during photosynthesis?
H2O → NADPH → Calvin cycle
n photosynthetic cells, synthesis of ATP by the chemiosmotic mechanism occurs during ________.
photosynthesis and respiration
What statement best describes the relationship between photosynthesis and respiration?
Photosynthesis stores energy in complex organic molecules; respiration releases energy from complex organic molecules.
Codes for a protein.
mRNA
Is a carrier for amino acids and has an anticodon
tRNA
Along with proteins, makes up a ribosome.
rRNA
This occurs when a cell divides to form two nuclei that are genetically identical.
is true for mitosis only
Homologous chromosomes synapse and crossing over occurs
is true for meiosis I only
Centromeres uncouple and chromatids are separated from each other.
is true for mitosis and meiosis II
Independent assortment of chromosomes occurs.
is true during Metaphase and Anaphase for meiosis only
Due to the use of RNA primers in DNA synthesis, the lagging strand of DNA requires extra attention to be sure
telomeres do not shorten with each DNA replication
Breaks hydrogen bonds between DNA nucleotides to separate the two strands of DNA.
Helicase
Adheres to DNA to keep complementary base pairs from re-annealing with one another
Single-Stranded Binding Proteins
Breaks covalent bonds between nucleotides to relieve the stress of strands twisting.
Topoisomerase
E. coli cells grown on 15N medium are transferred to 14N medium and allowed to grow for two more generations (two rounds of DNA replication). DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment?
one low-density and one intermediate-density band
Semiconservative replication involves a template. What is the template?
one strand of the DNA molecule
Suppose you are provided with an actively dividing culture of E. coli bacteria to which radioactive thymine has been added. What would happen if a cell replicates once in the presence of this radioactive base?
DNA in both daughter cells would be radioactive.
Shell color in clams is thought to be controlled by three different genes (each with two alleles). Shell coloration runs a range between pure white and dark purple. The addition of dominant alleles makes the shells more purple in coloration, while the addition of recessive alleles makes the shells more white. The majority of clams have shells that are medium purple, whereas the other colors are present, but in fewer numbers. This is an example of:
Quantitative traits
As electrons are passed through the system of electron carriers associated with photosystem II, they lose energy. What happens to this energy?
It is used to establish and maintain a proton gradient.
In autumn, the leaves of deciduous trees change colors. This is because chlorophyll is degraded and ___
carotenoids and other pigments are still present in the leaves
Plants photosynthesize _____
only in the light but respire in light and dark
Radish flowers may be red, purple, or white. A cross between a red-flowered plant and a white-flowered plant yields all-purple offspring. The flower color trait in radishes is an example of which of the following?
incomplete dominance
The fact that all seven of the pea plant traits studied by Mendel obeyed the principle of independent assortment most probably indicates which of the following?
All of the genes controlling the traits behaved as if they were on different chromosomes.
In cats, black fur color is caused by an X-linked allele; the other allele at this locus causes orange color. The heterozygote is tortoiseshell. What kinds of offspring would you expect from the cross of a black female and an orange male?
tortoiseshell females; black males
In certain plants, tall is dominant to short. If a heterozygous plant is crossed with a homozygous tall plant, what is the probability that the offspring will be short?
0
Duchenne muscular dystrophy is a serious condition caused by a recessive allele of a gene on the human X chromosome. The patients have muscles that weaken over time because they have absent or decreased dystrophin, a muscle protein. They rarely live past their twenties. How likely is it for a woman to have this condition?
One-half of the daughters of an affected father and a carrier mother could have this condition.
The combination of alleles that an organism inherits.
Genotype
The outward appearance of an organism based on inherited DNA sequence.
Phenotype
A particular eukaryotic protein is 300 amino acids long. Which of the following could be the maximum number of nucleotides in the DNA that codes for the amino acids in this protein?
900
RNA is created in a ________ fashion, which means DNA is read from _
5’ to 3’ / 3’ to 5’