exam 3 Flashcards
what is a primary risk factor for gingivitis, inflammatory periodontal diseases, and dental caries
dental biofilm
what is an integral aspect of the dental hygiene diagnosis?
assessment
heterotrophic microorganism that can live and grow in the presence of free oxygen, some are obligate, others facultative
aerobe
heterotrophic microorganism that lives and grows in complete( or almost complete) absence of oxygen; some are obligate, others facultative
anaerobe
dynamic, complex, multispecies communities of microorganisms that colonize the oral cavity/ unique characteristics allow biofilms to adapt to a variety of every changing environments; characteristics include: tenacious adherence to surfaces, protective EPS, three-dimensional structures with complex nutrient and communication pathways
biofilm
white or cream-colored “cheesy” mass that can collect over dental biofilm on unclean, neglected teeth; it is composed of food debris, mucin, bacteria sloughed epithelial cells.
materia alba
a thin acellular tenacious film formed of proteins, carbs, and lipids
acquired pellicle
where does pellicle tend to form
over exposed tooth surfaces and prostheses; pellicle is positioned as the interface between the tooth surfaces and the oral environment
where is pellicle thickest
near the gingival margin and areas undisturbed by the activities of chewing, swallowing, and speaking
how fast does pellicle form?
within minutes after eruption or after all soft and hard deposits have been removed from the tooth surfaces; fully formed within 30-90 minutes
what is composition of pellicle
glycoproteins that are selectively absorbed by the hydroxyapatite of the tooth surface
initial attachment of bacteria to the pellicle is by _____ ______ of microorganisms that originate from the oral mucosa
selective adherence
what has a high affinity for the hydroxyapatite tooth surface and initiate the process of pellicle formation
salivary proteins
____ _____ is continuous with the supragingival pellicle and can become embedded in tooth structure, particularly where the tooth surface is partially demineralized or rough from iatrogenic dentistry
subgingival pellicle
along with tooth surfaces, biofilm communities also form on :
oral mucosa, surfaces of tongue, and tonsils; their presence especially on the tongue and tonsils may contribute to malodor
during biofilm formation, organisms that colonize within the first few hours and primarily what kind?
gram positive cocci and rods
stages of biofilm formation: (5 stages)
formation bacterial multiplication and colonization matrix formation(EPS) biofilm growth maturation
in stage 4 of biofilm growth, enhancement of cell to cell communication process takes place, what is that called
quorum sensing
how is quorum sensing activated?
by specific genes located on the surface of bacterial cells within biofilm
what stage of biofilm formation is this? bacterial colonies mature and release planktonic cells to spread and colonize other areas within oral cavity; bacteria can disperse as single cells or in clumps
maturation stage 5
the pellicle, EPS, biofilm architecture, and resulting environment promote what organisms
anaerobic gram-negative bacterial growth activity
what day in biofilm formation is this? Early biofilm consists of primarily gram + cocci; streptococci, which dominate the bacterial population, include streptococcus mutans and streptococcus sanguis
day 1-2
what happens day 2-4 of biofilm formation?
- cocci still dominate, more gram + filamentous form and slender rods join surface of cocci colonies
- more filamentous forms grow into the cocci layer and replace many of the cocci
- people who form biofilm slowly will exhibit more cocci and fewer filamentous forms
mixed flora appears comprised of rods, filamentous forms, and fusobacteria
-biofilm near gingival margin thickens as more mature flora develops; gram - spirochetes and vibrios proliferate; as biofilm spreads coronally, newer/younger biofilm primarily coccal
days 4-7