Exam 3 Flashcards
How do organism use the acquired nutrients?
Amino acids get used up
Carbohydrates and lipids get burned as fuel
Energy is briefly stored as ATP
Energy is released
ADP and the third phosphate group is removed
Energy is consumed
ATP third phosphate group is added
Energy is used for membrane transport, cellular movement
Glucose for through why kind of reaction
Combustion
Combustion process
Burning glucose with oxygen to make carbon dioxide and water
Glucose to CO2 will releases energy and some energy is stored by taking ADP and phosphate and making ATP
Carbon atoms are oxidized during ADP+PO4 becoming ATP
Loss of electrons
Oxygen is more electronegative than carbon
Keeps more of the shared electrons
Carbon loses electron density
We need to count the number of bonds to oxygen
C 4 hydrogen most reduced carbon
C four bond o. Is the most oxidized
Oxidation
Loss of electrons
Reduction
Gain of electrons
Where is carbon oxidized
In cell metabolism
Every oxidation must be followed by a
Reduction
Biologically done by a coenzyme
Vice versa
NAD + IS
Oxidized
A small molecule that is derived from a small B vitamin
NADH is
Reduced
Also has a free floating proton
Coenzyme
Small molecule that is necessary for enzymes to work
Bucket analogy with NAD and NADH
When the bucket is empty it is NAD
And when it is full with 2 protons it is NADH
When NADH is reduced it is
High energy
Due to the 2 electrons carrying the energy with them
Second coenzyme
FAD oxidized AND FADH2 reduced
Bucket analogy
FADH2 high energy
FAD low energy
Aerobic cellular respiration
Glycolysis
Transition step
Citric acid cycle
Electron transport
Chemiosmosis
Glycolysis
Glucose with 6 carbon dioxide
Taking NAD and FAD(oxidized) to NADH and FADH2 (reduced)
-DH (ADP) to -AD(ATP)
Follow the carbon atoms
Follow the energy
Glycolysis properties
Sugar cutting
Universal metabolic pathway
Cytosol
Anaerobic
Cut glucose and partially oxidized
Makes ATP and NADH
Metabolic pathway
Series of enzyme linked reactions
Moving metabolically
10 steps
To go from glucose to 2 pyruvate
Investment phase
Taking ATP and using it
Taking 6c and cutting it into 2 3c
Payoff phase
Making 2 ATP and high energy 2 NADH
Typically glucose enters through
GLUT (facilitator transporter-follows it’s concentration gradient)
Step 1 glucose is phosphorylated by hexokinase 
Once, in the cytosol hexokinase is a first enzyme to initiate glycolysis
Taking glucose and an ATP and making glucose 6 phosphate plus ADP
EXERGONIC REACTION due to ATP investment
Glucose six phosphate is trapped in the cytosol, and can’t exit through GLUT
Kinase
Enzyme that move around phosphates
Step 2 glucose 6 phosphate to fructose 6 phosphate
Aldehyde to ketone (movement of where the double bone is placed between carbon and oxygen
Step 3 fructose 6 phosphate is phosphorylated again
We add another phosphate to fructose 6 phosphate
Making the reaction fructose 6 phosphate plus ATP goes = fructose 1,6 biphosphate plus ADP
EXERGONIC
Step 4 cutting (lysis step)
Fructose 1,6 bisphosphate is cut down the middle which produces 2 different 3carbon compound
Step five
Convert molecule with double bond in the middle with the molecule that has double bond at the end
Payoff phase overall
Everything is done twice per glucose
Extracting energy out
Glycolysis input
Glucose
2 ATP
4 ADP
2 NAD
Glycolysis output
2 pyruvate
4 ATP
2 ADP
2NADH
Glycolysis “follow the energy”
Net input: glucose, 2ADP,2NAD ENERGY COMES FROM GLUCOSE
Net output: 2 pyruvate,2 ATP, 2 NADH ENERGY LEAVES
Transition step
Pyruvate oxidation
Move carbon atoms from cytosol into mitochondria
Carbon is oxidized
One CO2 released per pyruvate
Mitochondria are complicated organelles
Two independent phospholipid bilateral; outer and inner membrane
More inner membrane than outer membrane
Three spaces: cytosol inter-membrane space, matrix
Pyruvate dehydrogenase in the mitochondria
Spans between the outer membrane and inner membrane
Big enough protein to cross two phospholipid bilayers
Acts as a transporter for pyruvate into the matrix
Twice per glucose
PD cuts pyruvate (cutting carbon 3 which gets oxidized) into acetly group
CO2 moves fast through phospholipid bilayers diffuse across mitochondrial membrane out into the blood
NAD+ is reduced to NADH
Because acetyl is highly active
Gets attached to a handle CoA temporarily chaperone that prevents acetly acid from doing unwanted reactions
Follow the carbon atoms pyruvate dehydrogenase
3c pyruvate to CO2 and AC-COA (2C)
Follow the energy pyruvate dehydrogenase
Pyruvate to NADH
Citric acid cycle general
Happens in mitochondrial matrix
Finish oxidizing carbon atoms
Store energy in reduced coenzymes
Cyclic metabolic pathway
Also called tricarboxylic acid cycle or Krebs cycle
Citric acid cycle
4c molecule that uses AC-COA , COA is released 6c compound and so on and so fourth individual carbon units are getting lost to CO2 within those individual units NAD turns to NADH
EVENTUALLY you’ll do GTP AND GDP AND THEN you’ll oxidize FAD TO FADH then back NAD and NADH
Step one citric acid
Starting with 4c compound oxaloacetic acid and acetyl COA
Citrate synthase takes OAA and takes carbon from acetly COA to make citrate acid and CoA
Citric acid cycle GDP to GTP
Energy is consumed to make an extra phosphate
Guanosine di phosphate and guanosine triphosphate
GTP turns to ATP by removing high energy phosphate and gluing it on ATP
Citric acid cycle
Input AC COA 2c per pyruvate, 2pyruvate per glucose
Output two co2
Citric acid follow the energy
Input: acetyl group
Output; 3NADH
1 FADH2
1 ATP OR GTP
electron transport and chemiosmosis will be placed together
as oxidative phosphorylation
goal of oxidative phosphorylation
re-oxidize coenzymes and transfer energy to ATP
where does electron transport take place
in the mitochondrial inner membrane
electron transport chain then
re-oxidizes the coenzyme which allows the citric acid cycle to continue to the chemiosmosis
within electron transport, energy is not transferred as ATP but as
proton electrochemical gradient
where does the citric acid cycle take place?
in the matrix
Complex 1
multiprotein complex embedded in the membrane that receives NADH (2 high energy electrons) as a transmembrane protein
complex one
once NADH brings the 2 high energy electron to the transmembrane protein, then it takes the 2 high energy electrons and turns NADH to NAD+ as a result,
Complex 1 is then reduced (temporarily holds to 2 electrons)
NAD+ then goes to the Citric Acid cycle or it wont continue.
what is necessary for the citric acid cycle to function
NAD+
Complex 1 passes 2 electrons to COQ
Phospholipids draw static move CoQ due to their tails moving side by side
COQ and Complex1 fit together. COQ picks up the 2 electrons. As a result, CoQ is now reduced and Complex 1 is oxidized. CoQ and 2 electrons drift away from complex 1 (fluid mosaic model)
CoQ and the 2 electrons
fluid mosaic lateral drift eventually leads COQ to complex 3. CoQ docks 2 electrons to complex 3. Oxidized CoQ and reduced Complex 3.
CoQ goes back to complex 1.
Complex 3 and Cytochrome C
Cytochrome C moves along the phospholipid bilayer reducing complex 3 and oxidizing cytochrome C. Cytochrome C then moves along to Complex IV.
Complex 4
picks up 2 electrons from cytochrome C.
Complex 4 passes electrons to oxygen. ONLY PLACE WE NEED OXYGEN
Oxygen picks up to electrons and makes molecular water.
how we breathe
oxygen picks up 2 electrons from complex 4.
serves as a terminal electron sync (Picks up electrons that are used already)
Electron transport
high energy NAD to complex 1-complex 4 to low energy electrons in water
why don’t the electrons go backwards?
NADH is extremely high molecule, so free energy (G)
as we pass the electrons to complex 1 the energy lowers and so on so forth
Electrons are losing energy as they move along the electron transport chain
if they went backwards , there would have to gain a lot of energy but since there is no pump to give energy the flow continues an exergonic way of energy
What happened to that energy
neither created nor destroyed, the energy is used to move proton. (active transport) Complex 1 moves proton