Exam 2 Selective Media Flashcards

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1
Q

MSA selects for:

A

Staphylococci and some other Gram-positive species that can withstand high salt concentrations

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2
Q

MSA inhibits:

A

Gram-negative growth

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3
Q

Inhibitor in MSA

A

Sodium Chloride

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4
Q

MSA substrate

A

Mannitol

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5
Q

MSA Indicator

A

Phenol Red

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6
Q

Positive result on MSA

A

Media turns yellow if mannitol fermentation occurs (generally S. aureus)

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7
Q

Negative result on MSA

A

Media is red

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8
Q

MAC selects for:

A

Gram-negative enteric species

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9
Q

MAC inhibits:

A

Gram-positive non-enteric species

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10
Q

MAC Inhibitor

A

Bile Salts and Crystal Violet

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11
Q

MAC substrate

A

Lactose

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12
Q

MAC Indicator

A

Neutral Red

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13
Q

MAC Positive result

A

Pink/red growth if lactose fermentation occurs

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14
Q

MAC negative result

A

Colorless or yellow growth if no lactose fermentation occurs

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15
Q

EMB selects for:

A

Gram-negative enteric species

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16
Q

EMB inhibits

A

Gram-positive species

17
Q

EMB inhibitor

A

Eosin Y and Methylene Blue

18
Q

EMB substrate

A

lactose

19
Q

EMB indicator

A

Eosin Y and Methylene Blue

20
Q

EMB positive results

A

Growth with green sheen means vigorous lactose fermentation; purple/pink growth means weak lactose fermentation

21
Q

EMB negative results

A

Colorless growth means no lactose fermentation

22
Q

HE selects for

A

Gram-negative enteric species

23
Q

HE inhibits

A

Gram-positive species

24
Q

HE inhibitor

A

Bile salts

25
Q

HE substrates

A

Sodium thiosulfate, fermentable sugars

26
Q

HE indicators

A

Ferric ammonium citrate, bromothymol blue, acid fuchsin

27
Q

HE positive results

A

Orange growth means lactose fermenter, black growth means sulfur reducer (mainly salmonella)

28
Q

HE negative results

A

no growth or colorless growth