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BIOL458 - Microbiology Lab > Exam 2 Differential Media > Flashcards

Exam 2 Differential Media Flashcards

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Biology 101 flashcards
1
Q

Blood Agar tests for

A

Hemolysis type

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2
Q

Blood agar substrate

A

Blood

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3
Q

Blood agar indicator

A

Blood

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4
Q

Blood agar positive result

A

Media clears for beta hemolysis, color change for alpha hemolysis

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5
Q

Blood agar negative result

A

Growth with no color change is gamma hemolysis

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6
Q

Chocolate agar tests

A

Fastidious organisms

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7
Q

Chocolate agar substrate

A

lysed RBCs

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8
Q

Chocolate agar positive result

A

Growth on chocolate agar and not lb agar means fastidious

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9
Q

Chocolate agar negative result

A

Growth on both means not fastidious

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10
Q

Phenol Red Broth tests for

A

fermentation, deamination, and gas production

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11
Q

Phenol red broth substrate

A

Lactose

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12
Q

Phenol red broth indicator

A

Phenol Red, Durham tube

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13
Q

Phenol red positive result

A

Yellow broth means fermentation, pink broth means deamination, and bubble means gas production

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14
Q

Phenol red negative result

A

red broth means nothing

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15
Q

MR-VP tests for

A

differentiates between mixed-acid pathway and 2,3-butanediol pathway for fermentation

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16
Q

MR-VP substrate

A

glucose

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17
Q

MR-VP indicator

A

Methyl red, VP reagents

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18
Q

MR-VP positive result

A

Red is positive for both; MR is mixed acid, VP is 2,3-butanediol

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19
Q

MR-VP negative result

A

colorless

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20
Q

MR-VP added reagents

A

MR is methyl red; VP is VP reagents A and B

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21
Q

Catalase test tests for

A

Catalase

22
Q

Catalase substrate

A

H2O2

23
Q

Catalase indicator

A

O2 release

24
Q

Catalase positive result

A

Bubbles

25
Q

Catalase negative result

A

no bubbles

26
Q

Oxidase test tests for

A

Cytochrome c oxidase

27
Q

Oxidase substrate

A

TMPD

28
Q

Oxidase indicator

A

TMPD

29
Q

Oxidase positive result

A

purple

30
Q

Oxidase negative result

A

colorless

31
Q

Nitrate Broth tests for

A

Nitrate reduction

32
Q

Nitrate broth substrate

A

Potassium nitrate

33
Q

Nitrate broth indicator

A

Durham tube, NItrate broth reagents A and B, zinc

34
Q

Nitrate broth positive result

A

bubble means denitrification, red color after A and B means nitrite formation, no color after everything means other nitrate reduction

35
Q

Nitrate broth negative result

A

red after zinc means no nitrate reductions

36
Q

NItrate broth added reagents

A

Nitrate Reagents A and B, zinc

37
Q

Citrate Agar tests for

A

Citrate metabolism

38
Q

Citrate agar substrate

A

Sodium citrate

39
Q

Citrate agar indicator

A

Bromothymol Blue

40
Q

Citrate agar positive results

A

Growth with blue color change or colorless growth

41
Q

Citrate agar negative results

A

no growth

42
Q

TSI tests for

A

Glucose or Lactose fermentation, sulfur reduction, gas production

43
Q

TSI substrate

A

Lactose, Sucrose, Glucose, Sodium Thiosulfate

44
Q

TSI indicator

A

Ferric Ammonium Citrate, Phenol Red

45
Q

TSI positive results

A

Yellow means fermentation, pink means deamination, black means sulfur reduction, space under butt means gas production

46
Q

TSI negative results

A

red means nothing

47
Q

SIM tests for

A

sulfur reduction, indole production, motility

48
Q

SIM substrate

A

Sodium thiosulfate

49
Q

SIM Indicator

A

Ferrous ammonium sulfate, Kovac’s reagent

50
Q

SIM positive results

A

Black means sulfur reduction, turbidity means motility, red Kovac’s reagent means indole production

51
Q

SIM negative results

A

colorless means nothing, clear means no motility

52
Q

SIM added reagents

A

Kovac’s reagent

BIOL458 - Microbiology Lab (3 decks)

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