Exam 2 Review Flashcards

1
Q

Transcription goes from ____ to _____

A

DNA to RNA

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2
Q

The human genome has _____ base pairs total and ____ from each parent

A

6 billion, 3 billion

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3
Q

The human genome has _____ protein coding genes

A

22,000

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4
Q

How many genes are there per million bc on the human genome?

A

7 genes, very spread out

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5
Q

The central dogma explains the connection between….

A

Genotype and phenotype

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6
Q

Genotype

A

DNA sequence (what’s encoded)

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7
Q

Phenotype

A

Presentation of proteins (what you can see)

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8
Q

During translation, the template strand is read…

A

From 3’ to 5’

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9
Q

Transcription creates _____ sequence that is _____ to the ____ strand and has the same sequence as the ____ strand.

A

ssRNA, complementary, template, coding

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10
Q

Transcription copies ____ sized sections (about ____ bp)

A

Gene, 2000

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11
Q

Gene expression can be regulated at _____

A

All levels

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12
Q

Not all genes are _____ in every cell

A

Transcribed

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13
Q

About ___ or ____ are expressed in every cell

A

1/2, fewer

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14
Q

Not all genes are transcribed in ____ _____

A

Equal amounts

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15
Q

RNA polymerase does not need a ____

A

primer

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16
Q

RNA polymerase uses _____ _____

A

Ribonucleotide triphosphates

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17
Q

Ribonucleotide triphosphates breaks off ___ _______ to form ______ _____

A

2 phosphates, phosphodiester bond

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18
Q

RNA polymerase….

A

Unwinds DNA in small transcription bubble (does not need help ask)

19
Q

RNA is _____ stranded and is much less ____

A

Single, stable

20
Q

RNA has an ____ on the ___ _____

A

-OH, 2’ carbon (instead of -H in DNA)

21
Q

RNA contains ____ instead of Thymine

22
Q

Certain RNA can ____ on itself via _____ _____ ______

A

Fold, complementary base pairing

23
Q

RNA folding via complementary base pairing improves ____ and is done by _____, ____, and ____ ____

A

Stability, tRNAs, rRNA, and splicing RNA

24
Q

The promoter sequence is ____

25
The promoter sequence is located….
Upstream of the txn start site
26
The promoter sequence binds….
Core or basal transcription factors
27
TATA box is located at ___ and is recognized by _____
-30, TBP (TATA binding protein - part of TFIID)
28
Dephosphorylated RNA polymerase is binded by…
the promoter sequence
29
The enhancer/switch is…
DNA
30
The enhancer/switch can be located….
Upstream or downstream
31
The enhancer/switch binds…
Activators (proteins) in response to environmental signals (transcription activators)
32
The mediator interacts with…
All transcription factors
33
The mediator is not always _____
Necessary
34
Once all factors are assembled, RNA Polymerase is _______, which begins _____
Phosphorylated, transcription
35
RNA polymerase transcribes until….
It falls off
36
RNA polymerase usually passes the ____ of the gene
End
37
____ strand of the DNA can be used as the template (but always in the ___ to ___ direction; depends on where the ____ is
Either, 3’ to 5’, promoter
38
_____ factors interact with ____
Remodeling, histones
39
Remodeling factors can shift _____, modify _____, or remove it ______
Nucleosomes, histones, completely
40
RNA processing improves _____
Stability
41
The 5’ cap is also known as the…
Methylguanine cap
42
The polyA tail is a sequence of….
Repeated A’s added to the end to protect from RNase
43
____ factors, ____ factors, and _____ factors attached to ____ ___ ___ and utilize attached ____ ____
Splicing, capping, PolyA, RNA pol tail, phosphate groups
44
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