Exam 2 (Ppt 3)

Question 1

____ _____ adds nucleotides to an existing ________ Jxn.

Answer 1

DNA polymerase

Primer:Template Jxn

Question 2

____ comes from the hydrolysis of phosphates of the incoming dNTPs

Answer 2

ENERGY

Question 3

What enzyme is involved in the hydrolysis of phosphates of incoming dNTPs which produces energy?

Answer 3

DNA polymerase (?)

Question 4

____ _____ have a THREE-DOMAIN STRUCTURE that contains 5′-to-3′ pol activity as well as 3′-to-5′exonuclease activity.

Answer 4

have a THREE-DOMAIN STRUCTURE(?) that contains 5′-to-3′ pol activity as well as 3′-to-5′exonuclease activity.

Question 5

What is contained in the active site of the DNA Pol?

Answer 5

2 METAL IONS

*They catalyze the rxn

Question 6

What protiens (enzymes) are at the replication fork?

Answer 6

Primase
DNA helicase
SSB (single strand binding protien)
Topo I/II (Topoisomerase)

Question 7

In what direction is the overall direction of DNA replication in?

Answer 7

same direction of leading strand pol. movement

OPP of lagging strand movement direction

Question 8

Model of Pol III holoenzyme contains

Answer 8

3 Pol III cores, sliding clamp, clamp loader + tau proteins

Replicates BOTH leading and lagging strands

Question 9

Tau proteins

Answer 9

a subunit that allows the cores to move independently of one another

Question 10

Trombone Model

Answer 10

1. LOOP of DNA b/w HELICASE + DNA Pol
2. TWO OKAZAKI fragments
3. Flexibility of t linkers and DNA

Question 11

New ____ _____ are synthesized PERIODICALLY during lagging strand synthesis.

Answer 11

RNA primers