Exam 2 --cofactors

Question 1

PLP or B6

Answer 1

1) aa degradation (gly, branched, met transulfuration)
2) aminotransferases/transamination (aa transfers NH3 to glutamate)
3) dopa–>dopamine

Question 2

B12

Answer 2

one carbon transfer–METHYL, needs IF to take B12 from diet

1) aa degradation (branched aa’s, met salvage)
2) degradation of odd chain fa’s… which is propionyl CoA–>succ CoA

Question 3

THF

Answer 3

one carbon transfer—FORMYL, METHYLENE, METHENYL, FORMIMINO, METHYL

1) aa degradation (gly, his/arg/gln/pro, met salvage)
2) pur/pyr synthesis

**Methotrexate blocks folate synthesis! (inhibits dihydrofolate reductase)

Question 4

Biotin

Answer 4

one carbon transfer in form of CO2

1) ACoA carboxylase
2) Pyr carboxylase
3) degradation of odd chain fa’s… which is propionyl CoA–>succ CoA

Question 5

TPP

Answer 5

1) PDH
2) alpha KG deh
3) transketalases in PPP
4) aa degradation (branched aa’s)

Question 6

UDP

Answer 6

1) GAG formation
2) glycogen synthesis
3) galactose metabolism