exam 2 - bootyaaayyy Flashcards
areabetterindicatorofhealthriskinsaltandfreshwater and can be used for detecting source contamination since they are very species specific
Enterococci
best recipe for cooking rabbit
definitely an exam question.
e.coli sterotype
O: 26 K: 60 F: 41 H: 11
T/F
animals have shigella
FALSE
Shigella spp.aretaxonomicallyalso what?
Escherichia
what are the cell associated virulence factors of e.coli
endotoxin
capsule
fimbrial adhesions
non fimbrial adhesions
extracellular virulence factors of e.coli
enterotoxins
cytotoxins
siderophores
what does e.coli cause in pigs
diarrhea
UTI
edema
MMA
causes watery diarrhea in piglets that are either neonatal, 2-4 weeks old, or weaning
ETEC
where are the adhesion factor receptors located for ETEC
in the small intestine
what are the adhesion factors of ETEC
F4(K88);F5(K99);F6(987P);F18;F41
where are the enterotoxins of ETEC located
on the plasmids
what is the heat liable enterotoxin of ETEC
LT
what are the heat stable enterotoxins of ETEC
Sta,STb,EAST1
an ETEC adhesion factor that is solely in neonatal pigs, and it is only expressed at body temperature
F4 (K88)
an ETEC adhesion factor that is in both bovines and swine, it is temperature dependent and involved in neonatal diarrheas
F5 (K99)
ETEC adhesion factor that is extrachromosomal and in neonatal pigs
F6(987P)
chromosomal ETEC adhesion factor that is rare but commonly found along with F5
F41
the ETEC adhesion factor that is involved with edema disease
F18
Non‐fimbrialadhesins of ETEC
AIDA‐I(adhesininvolvedindiffuseadherence)
in the small intestine
how would you inactive LT enterotoxin
Inactivated30minutes60ᵒC
T/F
LT has a low molecular weight
FALSE – high
**it is also a strong antigen wow
LT increases / decreases cAMP
INCREASES – it activates the adenylate cyclase
describe what happens to the Na+ and Cl‐ andHCO3+ with enterotoxin LT
Na absorption goes DOWN
Cl‐ andHCO3+ secretion goes UP
heat stable ETEC enterotoxin that causes activationofguanylatecyclasesystem
STa
**also causes sodium absorption to decrease and cl/hco to increase secretion
heat stable ETEC enterotoxin that causes prostaglandin E2 to go up and secretes water and electrolytes
STb
Stimulationof5‐hydrotryptaminesecretion is done by what heat stable enterotoxin of ETEC
STb
enteroaggregativeE.coliheatstabletoxin
EAST1 – closely related to STa
T/F
the ETEC virulence factors have agedependentimportance
TRUE
Which of these are hemolytic and which are non-hemolytic:
- neonatal ETEC
- neonatal til 4 weeks old ETEC
- weaning diarrhea
only neonatal is non-hemolytic the other two are hemolytic
which virulence factor enterotoxins does neonatal ETEC not contain
LT
EAST1
where does ETEC live in pigs
the intestines of healthy sows
what is the “equilibrium” of ETEC
Equilibriumbetweenmaternalimmunityandinfectionpressure
a break in this = disease :(
pathogenesis of ETEC
- Ascendingfromlowerbowel
- Oraluptake
- Smallintestine
- colonization
my piglet is drinking a lot of water but he has some really smelly poopy and its kinda watery. why
ETEC duh
in older pigs, what does the diarrhea of ETEC look like
more white/grey
less watery
how can you diagnose ETEC in piggys
Detectionofvirulencefactors
Agglutinationtests
PCR
how do you treat piglets with ETEC
Hydration(SC,IP)(PO,electrolytesolutionplusglucosesandaminoacids)
Antimicrobials(susceptibilityprofile!)
what do you do to prevent the ETEC
keepinfectionpressurelowandmaternalimmunityhigh
hygiene !
vax sows - LT
vax piglets - F4
select receptor free pigs
if you have a mixed ETEC and VTEC??
edema disease and watery diarrhea
edema disease in post weaning piglets
VTEC
what is the adhesion factor for VTEC and where is it located
F18
- *in small bowel
- specific receptor
T/F
if the receptor for F18 of VTEC is missing it will still adhere and stuff will make you sick
no it needs a specific receptor and there will be genetic resistance if it is absent
what are the two exotoxins that cause media necrosis in VTEC
VT2eorStx2e:
what age will F18 of VTEC start causing adhesion
10 days and on
T/F
the endotoxin of VTEC will cause acute mortality
TRUE – and shock
how is VTEC transmitted
sow to piglet
also weaning stress will cause a change in the intestinal floral – piglets will start to excrete it and infection pressure will go up contaminating other piglets
hyper acute toxin production of VTEC
called enterorrhagia – media necrosis
toxin production level of VTEC that causes permeabilitybloodvesselsoedema(CentralNervousSymptoms&dyspnea)
ACUTE
T/F
slower toxin production of VTEC will cause extravascular coagulation
FALSE – intravascular coagulation
when will you start seeing VTEC symptoms
older than 3 weeks old
*multiple piglets will be affected
clinical signs of VTEC
- Acutemortality(some)
- Diarrhea(some)
- Anorexia
- edema
- Hoarsevoice
- Centralnervoussymptoms(CNS)
- Dyspnea
- Eyelidsswollen
how to treat animals affected by VTEC
Fasting(eliminationoftoxin)
• (increaseofintestinalperistaltic)
• Antimicrobialtherapy
how can VTEC be prevented in pigs
lower stress
starter feed before weaning
probiotics to help intestinal flora
select F18 receptor neg animals
rare e.coli and causes diarrhea in pigs at weaning
EPEC
what gene do you isolate and use in PCR for diagnosis of EPEC
eae (intimin)gene
what is involved in the initial attachment for EPEC
BFP - bundle forming pili
by approx what age does intestinal e.coli in piglets seem to end
6 weeks
MostimportantpathogeninUTIinpigs(andothermammals)
E COLI
symptoms of UTI in pigs
Anorexia
Hematuria
Vaginalexudate
Generalmalaise(sometimes)
treatment of UTI in pigs
either culling or about 3 weeks of antibiotics
T/F
MMA and PPDS are both diseases in pigs caused by streptococcus
FALSE – caused be E COLI
what does MMA stand for
Metritis‐mastitis‐agalactiae
what does PPDS stand for
PostPartumDysgalactiaeSyndrome
other causes of MMA and PPDS besides E.COLI
hereditary
hormonal
feed
T/F
septicemia is normally a primary disease followed by enteric infection
FALSE – it is rarely primary and it is secondary to enteric infections
**pigs get this one from E.coli too wow
E.coli disease in bovine of less than 3 days old (neonates)
ETEC
e.coli diseases in bovine of older than a week old
EPECandEHEC
what does ETEC cause in cows
watery diarrhea and dehydration
NEONATES
what are the fimbriae virulence factors of ETEC in bovine
Fimbriae
• F5(mostcommon)
• F41(freqtogetherwithF5)
• F17(roleisnotclear)
• CS31A(relatedtoF4 and Freqinrelationtodiarrheainolderanimals
what are the toxin virulence factors of ETEC in bovine
Sta andStb
pathogenesis of ETEC in BOVINE
- Oraluptake
- Multiplication
- Enterotoxinproduction
- (Secondarysepticaemiae)
Equilibriumbetweenimmunityandinfectionpressure
**need colostrum!!!
how would you diagnose ETEC in bovine
ELISA
Agglutination and PCR
how could you do ETEC therapy treatment for COWS
separate sick animals
hydration
antibiotics
NSAIDS – for shock
*on milking farms separate calves from mom for hygiene and vax moms and get the colostrum to the baby
T/F
EHEC is zoonotic
TRUE
EHECandEPEC in bovine are mainly where in the body
large intestine
EHECandEPEC affect what age of cows and what does it cause
ages 1-8 weeks old
Mucoiddiarrhea+/‐ blood
prevention of mastitis by ecoli
hygiene
treatment of mastitis by ecoli
systemic antibiotics
list the virulence factors of mastitis by ecoli
NONE KNOWN HAHAH
what is the other name for septicemia by ecoli in bovines
ExPEC: NTEC
NTEC in bovine is a facultative pathogen?
YES
virulence factors of NTEC
- Serumresistance
- Ironuptakesystems
- Endotoxin
- Capsule
- Adhesionfactors(P,F17,AfaE‐VIII,CS31A)
- CNF1,CNF2toxins
prevention of septicemia in bovines
colostrum
what do you look for in the serum for diagnosing NTEC in bovine
low gamma globulins
T/F
you can give gamma globulins from older animals to young animals as a therapy method for NTEC septicemia in bovine
TRUE
Mostimportantbacterialdiseaseinbroilersandlayers
APEC
most frequent serotypes in birds and poultry
O1,O2,O78
how does e.coli disease in poultry and birds appear clinically
Clinicalappearances:
• Neonatal colibacillosis (septicaemia)
• Respiratory colibacillosis&septicaemiae
• Peritonitis (layers)
• Scabbyhip/necroticdermatitis(broilers)
what do the oldbackyardchickens get from e.coli disease
Coligranuloma
in e.coli disease in birds, Otitismediais part of what?
swollenheadsyndrome
how do birds get neonatal colibacillosis
egg shell contamination
neonatal contamination
manipulation of the chicks
** all lead to yolk rest infection - omphalitis –
causes sepsis and death or growth retardation
how do birds get Respiratorycolibacillosis
it is excreted in the feces and inhaled or there is damaged epithelium in the respiratory tract
T/F
Peritonitisis commonly seen in turkeys
FALSE – layer chickens
an endogenic infection that layers get at the start of a lay and it can be a chronic form in older birds
peritonitis
omphalitis
yolk rest infection