Exam 2

Question 1

To conversion of one mole of pyruvate to 3 mol of CO2 via pyruvate dehydrogenase and the CAC also yield _____ mol of NADH, _____ mol of FADH2 and ____mol of ATP(GTP)

Answer 1

4, 1, 1

Question 2

Which combination of cofactors is involved in conversion of pyruvate to acetyle CoA.

Answer 2

FAD, TPP and lipoic acid

Question 3

Which of the following reactions under standard condition is not highly exergonic ( ∆G’ᵒ ≤ - 10 KJ/mol )

Answer 3

the reaction catalyzes for succinyl CoA synthetase (#5).

Question 4

Which enzyme or enzyme complex with arsenite block.

Answer 4

α-ketoglutrate dehydrogenase complex

Question 5

Which of the following statement is true:

Answer 5

ATP activate a kinase which inactivate pyruvate dehydrogenase

Question 6

Which one of the following enzymatic activities would be ……(missing part)

Answer 6

α-ketoglutrate dehydrogenase complex

Question 7

Which cofactor not correctly matched with a group that it helps to transfer from one

Answer 7

Lipoate: -CO2

Question 8

Investigators at MCPHS have recently discovered a compound they have dubbed CACarin. If this compound is added to a mitochondrial preparation that is oxidizing pyruvate as a substrate, the concentration of acetyl-CoA begins to increase. Which of the following enzyme does this compound most likely inhibit?

Answer 8

citrate synthase

Question 9

The oxidative decarboxylation of α-ketoglutrate proceeds by means of multi-step reactions in which all but one of the following factors are required. Which one is not required?

Answer 9

Biotin

Question 10

When α-ketoglutrate is metabolized to oxaloacetate via citric acid cycle, how many ATP equivalents are formed per molecule of α-ketoglutrate.

Answer 10

7.5 ATP formed per molecule of α-ketoglutrate

Question 11

Reaction of citric acid cycle that produce an ATP equivalent:

Answer 11

Succinyl coA to succinate

Question 12

The following reaction ∆G= 29.7

L-malate →oxaloacetate

Answer 12

Can occur in a cell as a result of coupling to reaction catalyze by synthase.

Question 13

FAD occur in

Answer 13

Succinate dehydrogenase

Question 14

Anaplerotic reaction:

Answer 14

Produce oxaloacetate and malate to maintain constant level of citric acid cycle intermediate.

Question 15

Which of the following is not true about regulation of pyruvate dehydrogenase complex:

Answer 15

Elevated level of NADH increase the activity of the complex.

Question 16

Which of the following statement is true:

Answer 16

Elevated level of NADH decrease the activity of citrate synthase.

Question 17

Protein serum albumin:

Answer 17

Carry fatty acid from adipose tissue to organ.

Question 18

CH3 C12COH (not sure from the molecule) the molecules enter the B-oxidation and citric acid cycle:

1. How many ATP equivalent required for this reaction:
2. Moles of NADH formed:
3. Molecules of FADH2 formed:

Answer 18

1. 2 ATP
2. 6 + (7*3) = 27 ATP
3. 6 + (7*1) = 13 ATP

Question 19

Tetrahydrobiopterin is used in the oxidation of

Answer 19

Phenylalanine

Question 20

Clear sign of phenylketoneurea is:

Answer 20

Phenylalanine in urine

Question 21

Vitamin Niacin is synthesized from:

Answer 21

Tryptophan

Question 22

Which of the following Vitamins is synthesized by bacterial GI:

Answer 22

Vit B12

Question 23

Decrease in the level of ……. and ……. lead to decrease in the synthesis of DNA

Answer 23

Folate and Vit B12

Question 24

Controls amino acid catabolism:

Answer 24

Carbamoyl phosphate

Question 25

In the breakdown of nucleotide in the brain, amino group which is produced will

Answer 25

Be transported to the liver in form of Glutamine

Question 26

Urea cycle:

Answer 26

Requires 1.5 ATP equivalent

Question 27

180 Ib male receive 2500 Kcal/d how many grams of protein are needed?

Answer 27

Range depends on 10-35% Kcal/d of protein

Question 28

Table question about PDCAAS (slide No. 53 in AA oxidation handout)

Answer 28

Something in 50’s range (maybe 51) - answer is A