Exam 2 Flashcards
About how many species of bacteria cause plant diseases?
~100
Fungi or Bacteria? Smaller order of magnitude Structural features difficult to see Metabolically complex Much less terminology
Bacteria
Visible mass of single cells
Colony
3 shapes of bacteria
Coccus, rod, curved rod
Cocci can be found in which 5 morphological structures?
single, pairs (diplococci), chains, tetrads, clusters
Rods can be found in which 5 morphological structures?
Bacillus (single), flagellated bacilli, chains, palisades, filaments
Curved rods can be found in which 3 morphological structures?
Single curved rod, spirilla, spirochetes
Which morphological shape of bacteria has no cell wall and can have varying shapes?
Pleomorphic
Which type of bacteria has a cell wall with thin peptidoglycan layer between two membranes?
Gram negative
Which type of bacteria has a cell wall with a single membrane and a thick peptidoglycan layer?
Gram positive
Which class of bacteria has a cell membrane but no cell wall?
Mollicutes
What rigid framework is responsible for cell shape and protects the cell from environmental stress?
cell wall
What are the 4 steps of gram staining?
- Crystal violet
- Iodine
- Wash with alcohol/acetone
- Safranin counterstain
What are the 4 steps of the KOH test?
- KOH on slide
- Collect bacteria from 24-48 hr colony
- Stir in KOH 5-10 sec
- Lift loop
In the KOH test, which type of bacteria has viscous slime?
Gram negative
Which type of flagellated bacteria have a single tail?
Monotrichous
Which type of flagellated bacteria have a tail on each end?
Amphitrichous
Which type of flagellated bacteria have many tails?
Lophotrichous
Which type of flagellated bacteria have tails all around its perimeter?
Peritrichous
Which bacterial structure is short and its primary function is attachment?
Fimbria
Which bacterial structure is longer and has multiple types with varying functions?
Pilus
Which pilus functions in attachment to surfaces?
Type 1
Which pilus functions in movement on surfaces (without flagella)?
Type IV
Which pilus functions in attachment to other bacteria for gene transfer?
Conjugation (Sex)
Which pilus functions in interaction with the host?
HrP
Which type of bacteria requires oxygen?
Obligate anaerobe
Which type of bacteria grows with or without oxygen?
Facultative anaerobe
Which type of bacteria grows best in low-oxygen conditions?
Microaerophilic
Which type of bacteria cannot survive in the presence of oxygen because it is toxic to them?
Strict anaerobe
What method do bacteria use to reproduce?
Fission - NO SEXUAL REPRODUCTION
Where do bacteria keep “extra” DNA?
plasmids
How do bacteria communicate?
Cell signaling - they use receptors to identify what’s around them
When bacteria swarm/multiply to reach a critical concentration for survival, this is an example of
Density-dependent communication
What two things are required for a plant bacterial infection to occur?
Critical mass (quorum) and an opening
Bacteria do not have spores like fungi, so their ______ is disseminated.
Entire body
A specialized survival structure in bacteria
Endospores
Do plant pathogenic bacteria produce endospores?
NO
Which 5 places do bacterial plant pathogens survive?
In/on host tissues In/on alternate hosts In soil On surfaces In vector
What are 5 common disease symptoms of bacteria in plants?
Leaf spots & blights
Wilts
Rots
Galls, phyllody
What are 2 examples of bacterial signs?
Slime and ooze
Which bacterial plant disease is primarily found in rosaecous hosts, its main symptoms include flagging, shepherd’s crook, leaf & twig blight, and cankers on the main stem? Its signs include bacterial ooze from fruit, leaves, and stem cankers and it is one of the oldest known bacterial plant diseases?
Fire Blight - Erwinia amylovora
Gram Negative
What is the primary vector of fire blight?
Insects (bees)
Which bacterial plant disease is seedborne with symptoms including angular spots on leaves (initially water-soaked), deformation, necrotic margins, leaf drop, and small blister-like brown warty fruit spots?
Bacterial spot of pepper and tomato - Xanthomonas
Which bacterial plant disease has a wide host range and is pectolytic? It rots almost anything except the epidermis and its secondary bacterial colonization leads to a foul odor?
Soft Rots - Pectobacterium, Dickeya
Which bacterial plant disease is not harmful to humans, but causes fruit blotches on watermelon?
Watermelon fruit blotch - Acidovorax avenae citrulli
Which bacterial plant disease has a wide host range in dicots, is a soil inhabitant and its symptoms include gall formation near the soil line and girdling and plant death?
Crown gall - Agrobacterium tumefaciens
Which disease is a pathogen only because of the genes on its plasmid - it is not pathogenic on its own? What is the name of the plasmid?
Agrobacterium tumefaciens; Ti Plasmid
Which two plant genes are growth hormones whose overproduction causes galls in Agrobacterium tumefaciens?
Auxin, Cytokinin
Which bacterial plant disease is found in citrus plants and its symptoms include raised spots with oily, watersoaked margins and a yellow halo, defoliation, and early fruit drop?
Citrus canker - Xanthomonas citri
Citrus canker was introduced the first time in what year? The second time?
1910; 1986
The first time citrus canker was introduced, how long did it take to eradicate?
20 years
Which bacterial plant disease has a wide host range (solanaceae, banana, geranium, pothos), is a soil inhabitant infecting through the roots and moving upward through the xylem? Its symptoms include wilt and vascular discoloration.
Bacterial wilt - Ralstonia solanacearum
Which bacterial plant disease is a fastidious bacteria (picky eater), vector borne, and xylem-limited? Its symptoms include interveinal chlorosis, marginal necrosis, leaf drop, fruit shrivels, and plant death?
Pierce’s disease - Xylella fastidiosa
Which vector transmits X. fastidiosa?
Sharpshooters
Which bacterial plant disease is phloem-limited, vectored by psyllids and symptoms include chlorotic shoots, leaf mottling, small lopsided fruit, and dieback/tree death?
Huanglongbing - Candidatus Liberibacter (HLB, yellow dragon, citrus greening)
Disease cycle of HLB
Psyllid feeds on infected tissue Bacteria multiply in psyllid Psyllid feeds on healthy tissue Systemic spread in plant Symptom development
Which bacterial plant disease is a gram-positive filamentous bacteria whose primary hosts are potato, sweet potato, beets, carrots and radishes? Its symptoms include corky surface lesions and root rot in sweet potatoes.
Common scab, pox - Streptomyces
Which mollicute plant disease has a primary host of palms, is phloem-limited and its vector is planthoppers? It is not cold-hardy and has had a recent northward spread.
Palm Lethal Yellowing - Candidatus Phytoplasma palmae
Which 9 bacteria are gram negative?
Erwinia, Pantoea, Xylophilus, Acidovorax, Burkholderia, Ralstonia, Pseudomonas, Xanthomonas, Agrobacterium
Which 4 bacteria are gram positive?
Clavibacter, bacillus, clostridium, streptomyces
Motile by single polar flagellum, no pigment produced on nutrient agar, grows at 40 degrees C. One species, A. citrulli, causes watermelon fruit blotch
Acidovorax
Motile by 1-6 peritrichous flagella, when growing on carbohydrate-enriched media, the bacteria produce copious extracellular polysaccharide slime. Colonies are non-pigmented to light beige. Induced plant diseases known as crown gall, hairy root, and cane gall
Agrobacterium
Motile with lophotrichous flagella, plant pathogenic bacteria with the exception of one species produce fluorescent pigments
Pseudomonas
Motile with polar flagella, no growth at 40 degrees C, forms two colony types on media amended with glucose; one is mucoid and the other is dry
Ralstonia
Motile with 1 polar flagellum. When growing on glucose-enriched media, the bacteria produces copious extracellular polysaccharide slime. Colonies are yellow, causes leaf spots and vascular wilts.
Xanthomonas
Non-motile bacterium; nutritionally fastidious requiring specialized medium such as glutamine peptone medium (PW); habitat is xylem of plant tissues
Xylella
Formerly Erwinia species; motile with peritrichous flagella; produce pectolytic enzymes that degrade pectate resulting in macerated plant tissue
Dickeya and Pectobacterium
Motile by peritrichous flagella; are non-pectolytic and the type strain E. amylovora causes fire blight
Erwinia
Motile by peritrichous flagella; non-pectolytic, most produce a yellow pigment; Stewart’s wilt of corn
Pantoea
Straight or slightly curved slender rod; irregular and often club-shaped; obligately aerobic, non-motile, bacterial canker of tomato. Gram +
Clavibacter
Vegetative hyphae produce extensive mycelium, aerial mycelium forms chains of spores, produce wide variety of pigments, many produce one or more antibiotics. Potato scab incited by S. scabies is most studied, produces a toxin that is a major virulence factor.
Streptomyces
What is a nucleic acid containing genes and a protein coat?
Virus
What is a circular dsDNA containing genes and no protein coat?
Plasmid
What is a small circular ssRNA with no genes and no protein coat?
Viroid
What is a nucleic acid (could have genes, could have protein coat) and requires a host virus?
Satellite
4 Examples of Mobile Genetic Elements
Plasmid
Virus
Viroid
Satellite
A mobile genetic element may consist of which two things?
Genes and protein coat
What 3 things do all mobile genetic elements have in common?
They act as obligate parasites
They replicate independently of the host genome
They can affect the host phenotype
What is the size in cubic nanometers of a large virus?
6x10^5
What is the size in cubic nanometers of a small virus?
2x10^4