Exam 2 Flashcards
Define selective permeability
Allowing some substances to cross it more easily than others
What is the fluid mosaic model?
Used to demonstrate the membrane is a “mosaic” of various proteins dispersed within the bilayer, with only the hydrophilic regions exposed to water
True or False: Phospholipids within the plasma membrane can move within the bilayer
True
True or False: Proteins can not move within the bilayer
False
As temperatures cool, membranes switch from a fluid state to a …
Solid state
True or false: The fluidity of a plasma membrane is affected by the type of hydrocarbon tails in phospholipids
True
At lower temperatures, the Plasma Membrane is more fluid if…
Unsaturated hydrocarbon tails with kinks are present
The plasma membrane is less fluid if…
Saturated hydrocarbon tails are present
Define viscous
Less warm temperatures, cholesterol makes the membrane less fluid by restraining the movement of phospholipids
How does chloesterol effect membrane fluidity at warm temperatures?
cholesterol makes the membrane less fluid by restraining the movement of phospholipids
How does cholesterol effect membrane fluidity at low temperatures?
At low temperatures, it maintains fluidity by preventing tight packing
What are integral proteins?
- Penetrate hydrophobic core
- Often transmembrane proteins
- Located all over the membrane
What are peripheral proteins?
- not embedded in lipid bilayer
- appendages are loosely bound to the surface of the membrane
What are the six major functions of membrane proteins?
- transport
- enzymatic
- attachment to cytoskeleton and extracellular matrix
- cell-cell recognition
- intercellular joining
- signal transduction
What is cell-cell recognition?
The cell’s ability to distinguish one type of neighboring cell from another
What is the purpose of membrane carbohydrates?
To interact with surface molecules of other cells, facilitating cell-cell recognition
How are glycolipids formed?
Membrane carbs covalently bonded to lipids
How are glycoproteins formed?
Membrane carbs covalently bonded to proteins
Molecules that start on the inside face of the ER…
End up on the outside face of the plasma membrane
When is symmetrical distribution of proteins, lipids, and assorted carbs in the plasma membrane determined?
When the membrane is built by the ER and the Golgi apparatus
What causes selective permeability?
Membrane structure
What is selective permeability?
When a cell must exchange materials with its surroundings, a process controlled by the plasma membrane
Describe the permeability of hydrophobic molecules?
- Nonpolar
- Hydrocarbons, CO2, O2
- Pass through membrane easily
Describe the permeability of hydrophilic molecules?
- Polar
- Sugars, H20, C6H12O6
- Do not pass through the membrane easily
Describe the permeability of ions?
- Na+, K+
- A charged atom and its surrounding shell of water
- Require transport proteins
What are the three mechanisms of transport?
Active
Passive
Bulk
What is passive transport?
- Substances diffuse DOWN the gradient
- Does not require ATP
- Facilitated diffusion included
What is active transport?
- Substances move against gradient
- ATP required
- Includes ion pumps and cotransports
What is bulk transport?
-Includes exocytosis and endocytosis
Define Osmosis
Mechanism of passive transport
Diffusion of water across a semipermeable membrane
How is the direction of osmosis determined?
By a difference in total solute concentration
True or false: Water diffuses from region of higher to lower solute concentration
FALSE
Define tonicity
Ability of a solution to cause a cell to gain/lose water
True or False: cells without rigid cell walls have osmotic problems in either a hypertonic or hypotonic environment
True
How do cells without rigid cell walls maintain internal environment?
Osmoregulation
Define osmoregulation
Control of water balance
What is special about the protist Paramecium?
It is hypertonic to its pond environment and has a contractile vacuole that acts as a pump
Define hypotonic
- When solute concentration is less outside than inside the cell (cell gains water)
- A cell swells until cell wall opposes uptake
What is the term for a hypotonic plant cell?
Turgid
Define hypertonic
- When solute concentration is greater outside than inside the cell (cell loses water)
- Membrane pulls away from cell wall (defined as plasmolysis)
What is the term for a hypertonic plant cell?
Plasmolyzed
Define isotonic
When solute concentration is the same inside as outside the cell (no net water movement)
What is the term for an isotonic plant cell?
Flaccid
What is the optimal environment for an animal cell?
Isotonic
What is the optimal environment for a plant cell?
Turgid (hypotonic)
True or False: Cell walls do not help maintain water balance
FALSE
Define transport proteins
Allow passage of hydrophilic substances across a membrane and are specific for the substance they move
What are channel proteins?
Have a hydrophilic channel that certain molecules or ions can use as a tunnel
Define aquaporins
Facilitate passage of water
Define carrier proteins
Bind to molecules to change shape to shuttle them across the membrane
Why is facilitated diffusion passive?
The solute moves down its concentration gradient
What are the inputs and outputs of the sodium potassium pump?
3 Na+ out
2 K+in
1 ATP used
What kind of transport mechanism is the Sodium Potassium pump?
Active
What is membrane potential?
Voltage difference across a membrane
True or false: the inside of the cell is negative compared to the outside
TRUE
Membrane potential favor the passive transport of ____ into the cell and _____ out of the cell
cations, anions
What is the electrochemical gradient?
2 combined forces driving the diffusion of ions across a membrane
What are the two forces of the electrochemical gradient?
Chemical and Electrical
What is the chemical component of the electrochemical gradient?
The ion’s concentration gradient
What is the electrical component of the electrochemical gradient?
Effect of the membrane potential on the ion’s movement
What is the electrogenic pump?
A transport protein that generates the voltage across a membrane
What are the typical examples of the electrogenic pump?
Sodium potassium pump (In animals) Proton pump (plants, fungi, bacteria)
Define cotransport
Occurs when active transport of a solute indirectly drives transport of another solute
What is the proton pump
Plants generally use gradient of H+ ions generated by proton pumps to drive active transport of nutrients into the cell
What is bulk transport?
- Small molecules and water enter or leave the cell through the lipid bilayer or by transport proteins
- Large molecules such as polysaccharides and proteins cross the membrane via vesicles
Define exocytosis
- transport vesicles migrate to membrane, fuse with it, and release their contents
- addition of plasma membranes
True or False: Many secretory cells use exocytosis to export their products
TRUE
Define endocytosis
- The cell takes in molecules by forming vesicles from the plasma membrane
- loss of plasma membrane
True or False: Endocytosis is a reversal of exocytosis involving different proteins
TRUE
What are the three types of endocytosis?
Phagocytosis
Pinocytosis
Recepter Mediated
What is Phagocytosis?
“Cellular eating”
Cell engulfs a particle in a vacuole
What is Pinocytosis?
“Cellular drinking”
cell creates vessicle around fluid
What is receptor mediated endocytosis?
Binding of ligands to receptors triggers vesicle formation
How does phagocytosis occur?
A cell engulfs a particle by wrapping pseudopodia around it, and packaging it within a vacuole. The particle is digested after the vacuole fuses with a lysosome containing hydrolytic enzymes
How does pinocytosis occur?
- The cell gulps tiny droplets of extracellular fluid to tiny vesicles.
- It is not the fluid itself that is needed by the cell, but the mlcs dissolved in the droplets.
- Nonspecific in the protein substances it transports
Define Ligand
Any molecule that binds specifically to a receptor site of another molecule
How does receptor mediated endocytosis occur?
Enables the cell to acquire bulk quantities of specific substances, even though these substances may not be very concentrated in the extracellular fluid
Describe intracellular receptors
- Flourid in the cytosol/nucleus of target cells
- small hydrophobic chemical messengers can easily cross membrane and activate receptors
What are examples of intracellular receptors?
Steroid and thyroid hormones of animals.
What is the role of an activated hormone receptor?
It can act as a transcription factor, turning on specific genes
Describe Protein Phosphorylation and Dephosphorylation
- Signal is transmitted by a CASCADE of protein phosphorylations
- This phosphorylation and dephosphorylation system act as a molecular switch
What is the role of the protein kinase?
Transfer phosphate groups from ATP to a protein
What is the role of the protein Phosphatase
To remove the phosphates
Describe the response of the phosphorylation and dephosphorylation
- other pathways regulate the activity of enzymes
- At each step, the number of activated products is much greater in the preceding step
- ultimately a signal transduction pathway leads to regulation of one or more cellular activities
Define metabolism
- The totality of an orgnaism’s chemical reactions
- an emergent property of life that arises from interactions between molecules within the cell
A metabolic pathway
- Begins with a specific molecule and ends with a product
- Each step is catalyzed by a specific enzyme
What is an exergonic reaction?
- releases energy that can perform work
- spontaneous
- negative delta G
- catabolism
- Goes down on a graph
What is an endergonic reaction?
- Requires energy to perform work
- nonspontaneous
- positive delta G
- anabolism
- Goes up on a graph
What are the three types of work performed by the cell?
Chemical
Mechanical
Transport
How do cells perform work?
They manage energy resources by energy coupling