Exam 1 Flashcards
Define Scientific Method
Procedural steps used to outline an experiment
What are the steps to the Scientific Method?
- Identify problem
- Experiment (methods)
- Results
- Conclusion
Define hypothesis
Proposed explanation of why something is the way it is
Define Prediction
An “If-Then” statement
Define control group
Receives no or neutral treatment. Experimental group results are compared to this group.
Define independent variable
The variable that the researcher manipulates in order to obtain a result.
Define dependent variable
Variable affected by the manipulation of the independent variable.
What are the four large macromolecules?
Carbs, Lipids, Proteins, Nucleic Acids.
Define Carbohydrate
Serve as fuel and building material
What are Carbohydrates composed of?
Sugars and their polymers
What are the three types of sugars?
Monosaccharides, Disaccharides, Polysaccharides
What are the three types of sugars made up of?
One or more glucose held together by a glycosydic linkage
What kind of bond holds carbs together?
Glycosidic linkage
What are the two types of monosaccharides?
Glucose and Fructose
How are glucose and fructose similar?
They are hexoses and are isomers of each other
Define isomer
Same chemical formula, different structural formula
How are glucose and fructose different?
Glucose: Aldose, 6 member ring
Fructose: Ketose, 5 membered ring
What is the difference between a ketose and an aldose?
A ketose has a carbonyl group in the middle of the chain; An aldose has a carbonyl group at the end of the chain.
How do polymers form large molecules?
Dehydration reaction by removing a water mlc to form a new bond.
How do polymers disassemble?
Hydrolysis reaction by adding a water causing a bond to break.
What is the chemical formula for glucose?
C6H12O6
What is the difference between alpha and beta glucose?
In alpha glucose, first carbon has hydrogen on top. In beta glucose first carbon has hydroxide on top.
Can humans break down alpha or beta glucose?
Alpha glucose.
Define disccharide
Composed of two sugars
What are the three disaccharides?
Maltose, Lactose, Sucrose
Define sucrose
Glucose + Fructose
Define lactose
Glucose + Galactose
Define Maltose
Glucose + Glucose
Define Polysaccharide
Composed of 3 or more sugars
What are the four types of polysaccharides?
Starch, Glycogen, Cellulose, and Chitin
Define starch
A plant polysaccharide consisting of entirely glucose monomers. It is the storage form of glucose in plants
What are the simplest form of starch
Amylose and Amylopectin
How do Amylose and Amylopectin differ?
Amylose is unbranched. Amylopectin is branched.
Define glycogen
An animal polysaccharide consisting of entirely glucose monomers. Is the storage from of glucose in animals.
Define cellulose
Consists entirely of glucose monomers and is a major component of cell walls in plants
How do starch and cellulose differ?
They have different glycosidic linkages.
Starch= 1-4 linkage of alpha glucose, 3D, helical
Cellulose= 1-4 linkage of beta glucose, 3D, straight
Why can’t humans digest cellulose?
We lack the enzyme enabling our digestion of beta glucose.
Define Chitin
A structural polysaccharide found in the exoskeleton of arthropods.
How do lipids differ from the other macromolecules?
They are NOT true polymers
Are lipids hydrophilic or hydrophobic?
Hydrophobic
What are the three types of lipids?
Fats, Phospholipids, Steroids
What is the structure of Fats?
aka Triglyceride is glycerol + 3 fatty acids.
What is the function of fats in the human body?
Energy storage, cushioning, insulation.
How do fatty acids vary?
Length (#of carbons), #of double bonds, locations of double bonds
Define Saturated fatty acid
Have max # of hydrogen atoms
- No double bonds
- More common in animals
- Solid at room temperature.
Define Unsaturated Fatty acid
- Have one or more double bonds
- More common in plants
- Liquid at room temperature
What are saturated fats saturated with?
Hydrogens
How does a vegetable oil become a solid food product?
Hydrogenation (adding hydrogen) and creates a trans fat
What is the functional group structure of a phospholipid?
-Have 2 fatty acids and one phosphate group
What is the phospholipid structure?
A hydroPHILIC head and hydroPHOBIC tails.
Define Steroid
Lipids characterized by a carbon skeleton consisting of fused rings
What are the types of steroids?
Cholesterol, Testosterone, Estrogen
What are the 7 functional groups?
Hydroxyl, carbonyl, carboxyl, amino, phosphate, sulfhydryl, methyl
How do you identify the hydroxyl group?
R———OH
How do you identify the carbonyl group?
C=====O
How do you identify a ketone from the carbonyl group?
Carbonyl is in the middle of the chain
How do you identify an aldehyde?
Carbonyl is at the end of the chain
How do you identify the carboxyl group?
O
||
R—–C——-OH
How do you identify the Amino group?
H——N——H
|
R
How do you identify the phosphate group?
O || O- -------P------- O- | O
How do you identify the Sulfhydryl group?
R————SH
How do you identify the methyl group?
H | R-----------C------H | H
What type of bond holds lipids together?
Ester Linkage
Define Protein functions
Enzymatic, storage, hormonal, contractile/motor, defensive, transport, receptor, structural
Define polypeptides
Polymers of amino acids
Define the functional group structure of amino acids
Possess an amino group and a carboxyl group
How can amino acids differ?
Differ in properties due to differing side chains. These properties are Non-polar, polar, acidic, or basic.
What bonds an amino acid together?
Peptide bonds
How is a proteins function determined?
The specific conformation
What are the four structures of an amino acid?
Primary, secondary, tertiary, and quaternary
Define primary structure
Unique chain of amino acids in a poly peptide
Define secondary structure
- The folding/coiling of a polypeptide.
- Determined by BACKBONE and hydrogen bond interactions.
- Contains alpha helices (coil) and beta pleated sheet (folded)
Define tertiary structure
3D shape of a polypeptide, determined by SIDECHAIN interactions
Define Quaternary structure
Overall protein structure. Made when multiple peptides come together
What is an example of quaternary structure?
Hemoglobin
What determines protein conformation?
Physical and chemical conditions (pH and temp)
Define denaturation
When a protein unravels and loses its native conformation
All of the following are linked together by peptide bonds except: Hemoglobin Collagen Transport Protein Cholesterol Insulin
Cholesterol
Define gene
A unit of inheritance that programs an amino acid
What are the two types of nucleic acids?
Deoxyribose Nucleic acid (DNA) and ribonucleic acid (RNA)
What is the function of DNA?
Stores information for the synthesis of specific proteins
What are the three components of a nucleotide?
Pentose sugar, Nitrogeneous base, Phosphate group
Define a nucleoside
Portion of a nucleotide without the phosphate group
Define DNA
Deoxyribonucleic acid, Nitrogeneous bases form hydrogen bonds in complementary fashion.
How do you pair DNA?
A always with T
G always with C
Describe the DNA backbones
They run in opposite 5’ to 3’ directions referred to as antiparallel
What is the name of the monomer of a carbohydrate?
Monosaccharide
What is the name of the polymer of a carbohydrate?
Polysaccharide
What type of linkage is present in carbohydrates?
Glycosidic linkages
What type of component is present in a lipid?
Fatty acids
What type of larger mlc is present in a lipid?
Triaglycerides
What type of linkage is present in lipids?
Ester Linkages
What type of monomer is present in proteins?
Amino acids
What type of polymer is present in proteins?
Polypeptides
What type of linkage is present in proteins?
Peptide bonds
What type of monomer is present in nucleic acids?
Nucleotides
What type of polymer is present in Nucleic acids?
Polynucleotides
What type of linkage is present in nucleotides?
phosphodiester linkages
How do you identify a polar side chain?
Contains N,O,S, or an OH group
How do you identify a nonpolar side chain?
Only carbons and hydrogen
How do you identify an acidic side chain?
Negative charge on the side chain
How do you identify a basic side chain?
Has a positive charge on the side chain
Define cell
The simplest collection of matter that can live
Define Cell fractionation
Takes cell apart and separates the major organelles from one another based on size and density
Define centrifuge
Used to fractionate cells into their component parts
What are the two types of cells?
Prokaryotic and Eukaryotic
What are the domains of Prokaryotic cells?
Bacteria and Archea
What are the domains of Eukaryotic cells?
Protists, fungi, animals, and plants
What do both prokaryotic and eukaryotic cells share?
Ribosomes, plasma membrane, DNA, RNA, chromosomes, cytosol
What is in an animal cell but not a plant cell?
Lysosomes, centrioles, flagella
Define centrosome
Where microtubules are initiated, contain pair of centrioles
What is cytoskeleton function?
Reinforces cell shape, functions in cell movement, anchors many organelles
Microvilli
Projections, increase as surface area increases
Define Peroxisomes
produces hydrogen peroxide
Define Mitochondrion
cellular respiration, ATP generated
Define Lysosome
digestive organelle
What are in plant cells but not animal cells?
Chloroplasts, central vacuole, cell wall, plasmodesmata
Define cell wall
Outer layer, maintain cell shape, protects from cell damage
Define plasmodesmata
Channels through cell walls, connect with cytoplasms of adjacent cells
Define chlorpolast
Type of plastid, photosynthesis
Define nuclear laminae
lines the inner surface of nuclear envelope
Define nuclear envelope
double membrane, encloses the nucleus, separating its content from the cytoplasm
Define Nucleolus
synthesis of ribosomal RNA
Define chromatin
Composes chromosomes which compose DNA
What are the components of the endomembrane system?
Nuclear Envelope ER Golgi Apparatus Lysosomes Vacuoles Plasma Membrane
True or false: the ER accounts for more than half the total membrane in many eukaryotic cells
True
What are the 2 regions of the ER?
Smooth and rough
What are the functions of the smooth ER?
Synthesize lipids, metabolizes carbs, stores calcium, detoxifies poison
What are the functions of the rough ER?
Synthesize proteins (membrane and secretory)
Define cisternae
Flattened, membranous sacs
What are the functions of the Golgi Apparatus?
Modifies products of the ER, manufactures macromolecules, sorts and packages materials into transport vesicles
What are the two sides of the Golgi apparatus?
Cis and trans
What is a vacuole?
Membrane-bound sac with varied functions
What are the types of vacuoles?
Food vacuoles, contractile vacuoles, and central vacuoles
What is a food vacuole?
formed by phagocytosis
What is a contractile vacuole?
Pumps excess water out of cells
What is a central vacuole
holds organic compounds in water, breaks down waste
Where does Aerobic Respiration occur?
The mitochondria in plant and animal cells
What are the by products of Aerobic Respiration?
ATP, CO2, and H2O
Where does photosynthesis occur?
The chloroplast of a plant cell?
What happens during photosynthesis?
Light energy is converted to glucose and produces O2
How did mitochondria and chloroplasts come about?
A cell would engulf a another cell to obtain a mitochondria or chloroplast. That is why it is double membraneous
Define endosymbiont
Working together
Define cytoskeleton
A network of fibers extending throughout the cytoplasm
What are the 3 molecular structures composing the cytoskeleton?
Microtubules, microfilaments, intermediate filaments.
What are the fundamental properties of living organisms?
Order, Regulation, Growth & development, Energy utilization, Response to the environment, Reproduction, Evolution
What is the correct sequence of hierarchy downward?
Biosphere, Ecosystem, Community, Population, Organisms, Organ system, organ, organ tissue, cells, Organelles, Molecules
What do the dynamics of any ecosystem depend on?
Chemical cycling and Energy flow
What two main points did Darwin focus on?
Descent with modification and the evolutionary mechanism, and natural selection
What three observations is natural selection based on?
Variation, over production, adaptation
Define Variation
Individuals in a population vary in traits
Define Over Production
More offspring survive to reproduce causing organisms to compete for resources
Define Adaptation
Species generally suit their environment
Define Artificial Selection
Selective breeding of domesticated plants and animals by humans
What are the four elements essential to life?
Carbon, Hydrogen, Oxygen, Nitrogen
How many trace elements are there?
14
Define trace element
Elements required by an organism but only in minute quantities
Define isotope
An element that differs in the number of neutrons but has the same number of protons
Define valence electrons
The number of unpaired electrons in the outermost (valence) shell
When asking what atom has a valence of two….
look for the outer shell that needs two more electrons to complete the octet rule
What is the difference between a nonpolar and polar covalent bond?
Non polar the atoms have similar electronegativities, and share the electron equally.
Polar have different electronegativities and share the electron unequally.
When is chemical equilibrium reached?
When the forward and reverse reaction rates of a chemical are equal (Is balanced)
What are the four properties of water?
Cohesion, Temperature Moderation, Expansion upon freezing, Versatility as a solvent
True or false: Water has a high specific heat
True
What is an acid?
A proton donor
What is a base?
A proton acceptor
What are the 8 Hierarchies
Kingdom, Phylus, Class, Order, Family, Domain, Genus, Species
Does gamete formation occur in miosis or mitosis?
Miosis
Does cell division occur in miosis or mitosis?
Mitosis
How do you determine the pH based on the number of Hydronium ions given
10^-ph H=pH
ex// 10^-4=pH of four
In terms of hydronium ions is a pH of four acidic or basic?
Acidic
In termos of Hydroxide ions is a pH of four acidic or basic?
Basic
How do you find the pH based on the number of hydroxide ions given?
10^-xOH= 14-x=pH
How do you find the pOH based on the number of Hydroxide ions given?
10^-pOH=pOH
What is the largest component of the cytoskeleton?
Microtubules
What is the smallest component of the cytoskeleton?
Microfilaments
What are Microtubules made up of?
tubulin
What are microfilaments made up of?
Actin
What are intermediate filaments made up of?
Keratin
What do microtubules help function?
Cilia or flagella
What do microfilaments help function?
Muscle contraction
What hormone is used in cilia and flagella function?
dynein
What hormone is used in muscle contraction?
Myosin
What do intermediate filaments help function?
Cell shape
What are the functions of microtubules?
- cell division
- Chromosome movement
What are the functions of microfilaments?
- amoeboid movement
- cytoplasmic streaming
- microvilli
- cell division
What is the function of intermediate filaments?
nuclear lamina formation