exam 2 Flashcards
central dogma
dna — transcription — RNA — translation —- protein
coding strand
5’ - 3’
template strand
3’ - 5’
mRNA
5’ - 3’
palindromic sequence
inverted sequence
new nucleotide binds to
3’ end
purines
A, G
pyrimidines
T, C, U
leading strand
synthesis towards the fork
lagging strand
synthesis away from the fork
sticky ends
cut between base pairs mentioned
how RNA nucleotides differ from DNA
RNA uses ribose instead of deoxyribose, uses uracil instead of thymine
which are larger, purines or pyrimidines
purines
-35 region
TTGACA
-10 region
TATAAT
transcription start site
AATCGTGCAT - will start wherever the mRNA starts and is 10 nucleotides
shine-dalgarno
GAGG
translation start codon
ATG
substitution mutation
one nucleotide is replaced by another
lac operon
LacI LacP LacO LacZ LacY LacA