Exam 2 Flashcards
What are the 7 Proximate Analysis
Moisture
Ash
Nitrogen
Crude Fat
Crude Fiber
Gross Energy
Calcium and Phosphorus
What is Dry Matter?
How much moisture %
What temperature is Dry Matter heated to?
105C
What are the 2 formulas for calculating DM?
Final weight/ initial weight * 100 = DM
or
100 - % H2O = DM%
Why is DM important?
Most feed analysis is reported and conducted on DM basis
What is Ash
Estimate of inorganic material
What is the feed heated to and for how long?
Heat feed at 600 C for 6 hours in muffle furnace
What is the formula for Ash?
Starting weight - final weight
What are the two major method for calculating Nitrogen
Kjeldhal Method and Combustion Method
Protein contain what?
Nitrogen
Are all nitrogen compounds protein?
No
What is the formula for calculating crude protein
%=%N x 6.25
How do you extract Crude Fat
Ether extract, using ether or organic solvent to remove lipids.
What are 2 examples organic solvents?
Petroleum ether or ethanol
What is the is an important step when extracting fat
Must be air dry
Use extract
oven dry
Take final weight
What is crude fiber? Examples
All the fiber in the diet.
Hemicellulose, Cellulose
What must be removed before calculating crude fiber
Fat
What are the 3 steps to get the Crude fiber
- Boiling (refluxing) in a weak Acid
- Filtering and boiling in a weak Base
- Filtering and ashing
What is the formula to find crude fiber
(Extracted and acid/basic digested sample - ash residue)/ initial sample * 100 = %CF
What are the disadvantages for CF
low repeatability, some hemicelluloses are dissolved, protein can be bound in insoluble forms
What is NDF
Neutral Detergent Fiber
What is ADF
Acid Detergent Fiber
What is in ADF
Cellulose and Lignin
What is in NDF
Cellulose, Lignin and Hemicellulose
What is gross energy?
The total energy of everything in a sample
what instrument is used to determine gross energy?
Bomb calorimeter
How is digestible energy formed
Gross energy - Fecal energy loss
How is Metabolize Energy formed
Digestible energy - urinary energy loss
How is Net energy formed
Metabolized Energy - Heat increment
What is net energy?
Maintenance and production
What are two types of ash?
Dry and Wet
What is dry ash
ash with heat
Boil in HCl and nitric acid then dilute with pure water
What is wet ash
ash with perchloric acid
Boil in nitric acid and perchloric acid then dilute with water
What is the instrument used to find Ca
Atomic absorption spectrometer. The absorbance of the flames
What is the instrument used to find Phosphorus
Use colorimeter. The darker the color blue the more phosphorus
How do we find out how much feed is being utilized
Ileal Digestibility, takes feed in the ileum
What is TTU
Total Tract Utilization, takes feed from the feces
T or F: If one amino acid is missing, you cannot provide that protien
True
T or F: Feed ingredients contain various proteins that are digested to provide amino acids for the animal
True
T or F: Nutrient requirements are for amino acids, not CP
True
T of F: a diet which appears adequate in CP, can be deficient in one or more amino acids
True
Essential Amino Acid (EEA)
Essential amino acids cannot be produced or cannot be produced in sufficient quantity
Nonessential Amino Acid (NEEA)
They serve as a sources of carbon skeletons and amino nitrogen
Are NEEA necessary
There is a minimum amount of NEAA that are necessary in a diet
How are amino acid classified?
Based on the R group
Protein Structure
Primary
Secondary
Tertiary
Quaternary
What is primary structure
order of AA in the chain. Starting with methionine
What is secondary structure
local structure
What is tertiary structure
major folding over all shape of protein or subunit
What is quarternary structure
when multiple proteins come together
Order of protein digestion
Mouth
Stomach
Pancreatic secretion
What happens in the mouth
Mastication
What happens in the stomach
release of HCl and pepsinogen
What happens in pancreas
Proteolytic enzymes
What are the two types of Proteolytic enzymes
Endopeptidases and Exopeptidase
Endopeptidases converts Trypsinogen to what
Trypsin
Endopeptidases converts Chymotrypsinogen to what
Chymotrypsin
Endopeptidases converts Proelastase to what
Elastase
Exopeptidase converts Procarboxypeptidase A and B to what?
carboxypeptidase A and B
Where and How is AA absorbed? 3 steps
In the enterocyte
- Amino acid are absobred across the brush border membrane
- AA use the absorptive cell or transported into the blood stream
- the small peptides from the AA are transported to into the lumen side then through the blood side
Free Amino Acid
Most dependent on an Ion gradient
In 2 K+
Out 3 Na+
Di and Tri peptide absorption
Dependent on Na/K ATPase
What is limiting amino acid?
The amino acid found in the diet at the lowest concentration relatives to the animal’s requirement
How to formulate limiting amino acid?
inclusion AA / required AA * 100
Ex:
Lys req: 0.60
Given: 0.61
.61 Lys / 0.60 * 100 = 102%
Growing pigs Lys Req
Daily Lysine requirement = lysine (maintenance) + lysine (protein accretion)
Protein and Digestible Energy Relationship
Increase Digestible Energy intake increases protein gain
How is lys req expressed
lys:energy
Relationship between energy concentration and lys req
The lys requirement increases with increasing energy concentration in the diet
Relationship between ME and Feed Intake?
Increase Me, decrease Feed Intake
How is AA req expressed
Ratio of AA:Lys
What are the three concept of an ideal protein
- Must contain all EAA
- Closer to the AA profile to the tissue or product being produced, the more efficient it is
- High correlation between AA req for growth and carcass
Converting to Lysine Basis
% AA / % lys * % of Lys
Example
Threonine = .61/.95 * 100% = 64.2
What are the 2 ways to cover deficits in amino acid
Blending complimentary ingredients together or synthetic amino acids
What is bioavailability
the availabilty of nutrietnts for animal to absorbed
How to calculate bioavailability
What is absorbed / what is given * 100
How to calculate digestibility
(Amt of Nutrient in Feed - Amt of Nutrient in Feces) / Amt of Nutrient in Feed * 100