Exam 2 Flashcards
most common DNA is early years
semen and white blood cells
telogenic hairs
shed hair, no follicle present
reference sample
choose type of sample and collect under ideal conditions, buccal swab
chain of custody
keeps track of where sample is and who had the sample at all times
mixture
sample containing more than one type of sample, natural
contamination
sample that shouldnt be there due to human error from investigators, accidental
presumptive tests
use antibodies and chemicals to determine sample type, blood versus semen versus saliva
mRNA
messenger RNA that is less stable than DNA
aerosol barrier tips
used on micropiptees to prevent sample from contaminating the pipette and future sampels
nuclease inhibitors
EDTA and Mg++
proteinase K
DTT
chaotropic agents
proteinase k
digests keritan
DTT
breaks di-sulfide bridges
chaotropic agents
reduce h bonds
organic method
origional method, RFLP, need high quantity DNA, yiels dsDNA
denaturing agents in organic method
phenol
chlorophorm