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Bio 2 Lab > Exam 2 > Flashcards

Exam 2 Flashcards

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Biology 101 flashcards
1
Q

What is the gram stain appearance of Escherichia coli

A

Gram-negative bacilli in singles

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2
Q

What is the gram stain appearance of Serratia marcescens

A

Gram-negative bacilli in singles

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3
Q

What is the gram stain appearance of Bacillus subtilis

A

Gram-positive bacilli in chains

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4
Q

What is the gram stain appearance of micrococcus luteus

A

Gram-positive cocci in tetrads

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5
Q

What is the gram stain appearance of staphylococcus epidermidis

A

Gram-positive cocci in clusters

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6
Q

colony morphology of E.coli

A

white,moist colonies

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7
Q

colony morphology of serratia

A

red colonies

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8
Q

colony morphology of bacillus subtilis

A

dry feathery white colonies

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9
Q

colony morphology of micrococcus luteus

A

yellow colonies

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10
Q

colony morphology of staph

A

white colonies

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11
Q

colony morphology of chromo bacterium violaceum

A

purple/violet colonies

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12
Q

the first step (primary stain) in a gram stain procedure

A

crystal violet for 1 minute

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13
Q

the second step in a gram stain procedure

A

grams iodine for 1 minute

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14
Q

what else is grams iodine called? and why?

A

mordant, it makes the cell more receptive to staining

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15
Q

the third step in a gram stain procedure

A

95% ethanol

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16
Q

what does the alcohol do?

A

cause the crystal violet-iodine complex to leave the cell wall if the bacteria is gram negative

17
Q

the fourth step in a gram stain procedure

A

safranin red

18
Q

what is the technique that must be used EVERY time you are working with bacteria

A

aseptic techniques

19
Q

what is the cell wall of bacteria composed of

A

peptidoglycan

20
Q

what can be prescribed that works differently on gram positive or negative bacteria

A

antibiotics

21
Q

what two domains exist in the kingdom prokaryotae

A

Archaebacteria (ancient forms) and Eubacteria (modern bacteria)

22
Q

why do bacteria react differently to the gram staining process

A

because of the differences in their cell walls

23
Q

what is the cell wall structure of gram-positive bacteria

A

layers of peptidoglycan laying above the cell membrane and secured by amino acids

24
Q

what is the cell wall structure of gram-negative bacteria

A

cell walls stacked with a membrane layer- a layer of peptidoglycan and a top membrane layer

25
Q

what is a protist

A

a eukaryotic organism that is not a plant,animal or fungus

26
Q

why are protists classified as eukaryotes

A

they have a true nucleus

27
Q

what does an amoeba use for motility

A

psuedopodia (false foot)

28
Q

what is a unicellular fungi and what are they called

A

single-celled fungi, yeast

29
Q

what is “budding” in yeats

A

a type of asexual reproduction

Bio 2 Lab (2 decks)

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