Exam 2 Flashcards
What gives DNA its helical shape?
Plate stacking and Hydrogen bonding
In what way does a DNA strand grow?
5’-3’
In what way does the complimentary strand grow?
3’-5’
What are the steps in DNA replication for a leading strand?
- the DNA is unwinded or “unzipped” at an origin (replication fork)
- a short segment of RNA (primer) synthesizes and acts as a starting point for the enzyme DNA polymerase to attach to
- DNA polymerase then goes down the strand and replicates the strand with its matching base pair
- once replication is done RNA primers are then replaced with DNA by DNA polymerase
- any gaps left are then sealed together with enzymes
What are the steps in DNA replication for a lagging strand?
- the replication fork starts so that the DNA polymerase can bind and start replication
- a segment of RNA (primer) acts as a starting point for the DNA polymerase
- the DNA polymerase then has to keep unattaching and reattaching because it is moving away from replication fork instead of with the replication fork
- RNA primers have to continuously bind to act as starting points for replication, seeing as the polymerase keeps having to unattach and reattach, leaving it needing a new place to start
- once the lagging strand is replicated DNA polymerase then goes back and replaces the primers with DNA
- topoisomerase goes through and seals any nicks made to the strand during replication
What does topoisomerase’s function?
makes nicks in the double helix ahead of the replication fork to prevent it from getting too tightly wound up as the DNA is opened (releases tension), after replication it goes back and seals those nicks in order to avoid permanent damage
What keeps DNA polymerase 1 from floating away on the lagging strand whenever it has to reattach?
a protein known as the sliding clamp that holds DNA polymerase 3 molecules in place as DNA is synthesized, keeps the polymerase from floating away as it starts a new Okazaki fragment
What is an Okazaki fragment?
short sequences of DNA nucleotides which are synthesized discontinuously and later linked together by enzyme DNA ligase
What is DNA ligase’s function?
to replace the RNA primers with DNA sequences
What is a phosphodiester bond?
result of a condensation reaction between a hydroxyl group of two sugar groups and a phosphate group
Where is a phosphodiester bond found in the DNA strands?
5’ end phosphate group attaching to a 3’ end sugar
Why is the major groove where most contact is made in DNA rather than in the minor groove?
the minor groove is too narrow
What causes the DNA backbone to be highly charged and polar?
the phosphate groups
What gives the DNA helix its stability?
base stacking and Hydrogen bonding
How many rings do purines have?
2 rings
How many rings to pyridines have?
1 ring
A G-C pair is stabilized through how many Hydrogen bonds?
3
An A-T pair is stabilized through how many Hydrogen bonds?
2
What is a sequence of nucleotides on a single strand there for?
to code for the RNA which then codes for the primary protein
Do all RNA’s code for proteins?
no
What does ribosomal RNAs code for?
formation of the structure of the ribosome
What do tRNAs do?
deliver amino acids to translation, they DO NOT synthesize proteins
What are the non used regions between genes referred to as?
untranslated regions (UTR)
Describe what untranslated regions (UTRs) do.
they are sites where the enzymes will assemble to transcribe the gene
Is eukaryotic DNA packaged into a single chromosome or multiple chromosomes?
multiple chromosomes
What are specialized DNA sequences (regions) needed for?
DNA replication and chromosome segregation
True of False, the DNA chromosome is always highly condensed.
True
What are histones?
group of proteins found in chromatin
Are histones controlled?
yes, they are controlled
Are coding regions always continuous?
no, they can be fragmented
Why won’t molecular methods let the RNA out of the nucleus until it is fully correct?
because of the RNA’s nonlinear coding regions
Why would nonlinear coding regions mess up the whole DNA sequence?
there would be many regions where sequenced would be present that were not needed and that would make many unneeded amino acids
Is the process of duplication and segregation of chromosomes highly regulated? Why?
yes, because lack there of can be dangerous and could lead to many unwanted problems
What are the discreet sites where DNA replication starts called?
origin of replication
Do interphase chromosomes randomly occupy regions or are they in their own distinct regions?
own distinct regions
What causes premature aging in children?
the lamith protein
What is found in an interphase nucleus?
euchromatin
heterochromatin
nucleolus
How is the nucleolus formed?
by interaction of DNA, RNAs, and Proteins
What was the significance of beads on a string?
was an argument on how the basis of DNA has something to do with this specific formation
What is a histone octamer?
DNA wrapped around a protein core of 8 histone proteins
How many nucleotide pairs forms the histone octamer?
147
What happens on the tails of histones?
they are the site of post translational modification
What are the three forms of post translational modification?
acetylation, phosphorylation, and methylation
What causes the DNA to be 10,000 folds shorter than the full length?
Chromosome packing
What allows access to DNA in the regulation of chromosomes structure?
changes in the nucleosome structure
Do interphase chromosomes contain both highly condensed and more extended forms of chromatin?
yes
What else, other than changes in nucleosome structure, allows access to the DNA?
methylation and/ or acetylation of histone proteins
What makes heterochromatic and euchromatic regions change in the cell?
outside signals to the cell
What do histones control?
which regions of the chromatin are open for expression or not
Define Epigenetics.
control of expression that is not biases on the genetic sequences, rather it is based on heredity
What are polymerases 2 domains?
one goes down the strand lining up the template, the other finds mistakes and clips (edits) them
How many replication forks form at each replication origin?
2
What enzyme synthesizes DNA using a parental strand as the template?
DNA polymerase
Why does RNA have to prime the DNA before the polymerase can start replication?
because DNA polymerase can only bind to double stranded DNA
What enzyme replicates the ends of eukaryotic chromosomes?
telomerase
What acts as a replication machine during DNA synthesis?
a group of proteins
What enzyme unwinds the double stranded DNA to prepare it for replication?
helicase
What enzyme primes the single DNA strand with and RNA primer?
primase
How many times does the leading strand have to be primed for replication?
once
How many times does the lagging strand have to be primed for replication?
continually as it is being replicated
Do the two replication forks move in the same direction or opposite directions on the DNA strand?
opposite directions
What are the characteristics of DNA polymerase?
it polymerizes (replicates) and edits the strand it is working on
Where is one of the most common mutations that is involved in cancer?
P53
What enzyme joins the Okazaki fragments together on the lagging strand?
DNA ligase
What does ligase use to seal the nicks to give a continuous double strand?
ATP
What are telomeres?
short repeating sequences at chromosome ends
What does telomere shrinking raise the chances of?
cancer
What happens if the DNA is A-T rich?
the curve of the DNA is changed, it makes it more planar
What would happen without a special mechanism to replicate the ends of linear chromosomes?
some DNA would be lost during each cell division
Defects in telomerase can start allowing what to happen?
shorter DNA in turn wiping out genes after a period of time
What are some reasons for continual DNA damage in cells?
mutations, chemically induced errors, ionizing radiation induced errors
What is sickle cell anemia thought to be evolved for?
defense in certain areas of the world where malaria is prevalent
What enzyme requires general transcription factors in eukaryotes?
RNA polymerase
Where are mRNAs processed?
nucleus
Are the full DNA sequences transcribed into RNA or just portions of the full sequence?
portions of the full sequence
Define introns.
noncoding sequences between protein-coding genes
How are introns removed from pre-mRNA?
RNA splicing
Where are mRNA molecules eventually degraded?
cytosol
What are the chemical differences between RNA and DNA?
RNA uses ribose sugar while DNA uses deoxyribose sugar
RNA uses uracil while DNA uses thymine
What does RNA. polymerase do for DNA?
transcribes it into RNA
Can many molecules of RNA polymerase simultaneously transcribe the same gene?
yes
What are the different types of RNAs?
mRNA, rRNA, miRNA, tRNA, and other noncoding RNA
How does RNA polymerase know when to start and stop during transcription?
signals in the DNA
Where are promoter regions located?
upstream of gene sequence
Where are terminator regions located?
in the transcript
What is RNA polymerase 1’s transcribed genes?
most rRNA genes
What is RNA polymerase 2’s transcribed genes?
alll protein-coding genes, miRNA genes, and other noncoding RNA
What is RNA polymerase 3’s transcribed genes?
tRNA genes, 5S rRNA genes, and genes for many other small RNAs
What does RNA polymerase need in order to initiate transcription?
general transcription factors
Eukaryotic promoters contain sequences that promote what?
binding of the general transcription factors
Where are mRNAs processed?
in the nucleus
Where do processed mRNAs exit?
nuclear pores (arrows)
How are eukaryotic mRNA molecules modified?
5’ methyl capping and polyadenylation
Splicing is carried out by a collection of RNA-protein complexes called?
snRNPs (U1, U2, U6)
Do some pre-mRNAs undergo alternative RNA splicing to produce different mRNAs and proteins from the same gene?
yes
How are mRNA sequences decoded?
sets of 3 nucleotides
What does tRNA do in mRNA?
match amino acids to codons
Where are mRNA messages decoded?
on ribosomes
What signals the ribosomes to stop and start on the mRNA?
specific codons in the mRNA
What factors are used as antibiotics?
inhibitors of prokaryotic protein synthesis
What do ribosomes contain?
RNA, ribosomal RNA, and at least 40 different proteins
What are the 2 different parts of the ribosome?
small and large subunits
When do proteins start folding?
as they are synthesized
what reads the codons (nucleotide triplets) in transcription from RNA to protein?
anticodons
Why is the reading frame of nucleotides important?
the change or mutation of act reading Fram can cause the depletion of specific proteins needed, or make unneeded proteins
What is a point mutation?
where one nucleotide is added or removed
What shape do all tRNAs have?
L-shape
What do tRNAs couple with in order to correct the amino acids?
aminoacyl synthetase
Where are proteins produced?
on polyribosomes
What is the first amino acid on every protein?
methionine (Met)
How do ribosomes bind to the RNA for transcription?
the small subunits bind first, then the large subunit binds two the small subunit
What happens when a ribosome and protein reach the stop codon?
they disassociate from each other