EXAM 2 Flashcards

1
Q

Binary Fission

A

Main division of bacterial cells

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2
Q

Binarry Fission Process

A

Parent cell enlarges, duplicates its chromosome, and

forms a central transverse septum dividing the cell into two daughter cells

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3
Q

Lag phase

A

“flat” period of adjustment, enlargement; little growth

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4
Q

Exponential growth phase

A

a period of maximum growth when cells have adequate nutrients and a favorable environment

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5
Q

Stationary phase

A

rate of cell growth equals rate of cell death caused by depleted nutrients and O2, excretion of organic acids and pollutants

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6
Q

Death phase

A

As limiting factors intensify, cells die exponentially

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7
Q

Hemocytometer

A

Tool for counting the number of cells in a sample microscopically

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8
Q

Best time for gram straining

A

10-15 hours (young active synthesizing peptidoglycan)

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9
Q

Metabolism

A

all chemical and physical workings of a cell

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10
Q

Catabolism

A

degradative; breaks the bonds of larger molecules forming smaller molecules; releases energy

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11
Q

Anabolism

A

biosynthesis; process that forms larger macromolecules from smaller molecules; requires energy input

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12
Q

Enzymes

A

Biological catalysts

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13
Q

Enzyme function

A

Increase the rate of a chemical reaction by lowering the energy of activation

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14
Q

Apoenzyme

A

protein portion of conjugated enzymes or holoenzymes

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15
Q

Cofactors

A

nonprotein portion of conjugated enzymes or holoenzymes

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16
Q

Inorganic Cofactor Examples

A

metal ions - iron, copper, magnesium

17
Q

Organic Cofactor Example

A

Coenzymes (vitamins)

18
Q

Catalase Action

A

Breaks down hydrogen peroxide

19
Q

Oxidase Action

A

Adds electrons to oxygen

20
Q

Nitrate reductase

A

Reduces nitrate to nitrite

21
Q

DNA polymerase Action

A

Synthesis of DNA

22
Q

Botulinum toxin Action

A

Hydrolyzes protein needed for vesicle transport

23
Q

Catalase Required Cofactor

A

Iron

24
Q

Oxidase Required Cofactor

A

Iron, Copper

25
Q

Nitrate Reductase Required Cofactor

A

Molybdenum (Mo)

26
Q

DNA polymerase Required Cofactor

A

Zinc (Zn) and Mg

27
Q

Botulinum Toxin Required Cofactor

A

Zinc