Exam 1 Me

Question 1

H. influenzae

Answer 1

Needs chocolate agar to grow (RF p. 181)

Question 2

Chocolate agar

Answer 2

Different from SBAP

Question 3

BSL-1

Answer 3

Means…

Question 4

BSL-2

Answer 4

Means…

Question 5

BSL-3

Answer 5

Means…

Question 6

BSL-4

Answer 6

Means…

Question 7

Beta Hemolysis

Answer 7

Complete lysis of RBCs, leaving a clear zone around colonies

Organisms…

Question 8

Alpha Hemolysis

Answer 8

Partial lysis of RBCs, leaving greenish discoloration of culture medium surrounding colonies

Organisms…

Question 9

Gamma Hemolysis

Answer 9

Means, orgs

Question 10

N. lactamica

Answer 10

Discuss (RF p. 159)

Question 11

What enzymes contribute to staphylococcal virulence?

Answer 11

Coagulase, catalase, hyaluronidase, fibrinolysin, lipase, nuclease and penicillinase (aka β-lactamase)

Question 12

What toxins are produced by staphylococci, contributing to their virulence?

Answer 12

Cytotoxins (α, β, δ, γ leukocidin), exfoliative (epidermolytic) toxin, toxic shock syndrome, enterotoxin (A-F)

Question 13

What nonenzymatic and nontoxic virulence factors do Staphylococci have?

Answer 13

Slime production, capsule, cell wall (5.7 & 5.15)

Question 14

What does the agr do? Where is it found?

Answer 14

Quorum-sensing, changes expression… (5.16-5.17)

Question 15

General properties of staphylococcus

Answer 15

5.3 - 5.6

Question 16

MALDI TOF

Answer 16

2.20 - 2.24

Question 17

PFGE

Answer 17

2.15 - 2.16

Question 18

N. lactamica

Answer 18

In lab

Question 19

E. coli

Answer 19

In lab

Question 20

Candida sp.

Answer 20

In lab

Question 21

Group C streptococci

Answer 21

In lab

Question 22

Streptococcus bovis

Answer 22

In lab [and in slides presented in lecture, though not slides on Blackboard]