Exam 1 Lecture 3

Question 1

What is the type of Blood Specimen, Whole Blood?

Answer 1

Formed elements and fluid plasma remain combined (Sample does not clot)

Question 2

What is the type of Blood Specimen, Serum?

Answer 2

Blood clots (w/in 20-30 min) consisting of blood cells trapped in a mesh of fibrin- leaving serum

Question 3

What is the type of blood specimen, Plasma?

Answer 3

• Blood must NOT Clot (Antigocagulant added)

- Centrifugation forces RBCs down and leaves plasma on top

Question 4

Name the % of each of the following, Plasma, Formed elements in a blood specimen?

Answer 4

55% Plasma

45% Formed elements

Question 5

What is contained in the Plasma and Formed elements of a blood specimen?

Answer 5

• Plasma: Water 90%, Solutes 10%
• Erythrocytes
• Leukocytes and Thrombocytes (Platelets)

Question 6

What is the Plasma portion of a Blood Specimen?

Answer 6

Liquid portion of anticoagulated whole blood, contains all coagulation proteins

Question 7

What is the liquid portion of a clotted blood sample that lacks coagulation protein fibrinogen called?

Answer 7

Serum

Question 8

What is the most common specimen submitted to the laboratory? Give the %.

Answer 8

Venous Blood Samples

80% of Laboratory Workload

Question 9

What is the second most common specimen sample submitted to the laboratory?

Answer 9

Capillary Blood Samples

Question 10

What is the composition of a Capillary Blood Sample?

Answer 10

Mixture of

• Arteriole
• Capillary
• Venule Blood
• Tissue Fluid (Interstitial/Intracellular)

Question 11

When is Capillary Blood Specimen recommended?

Answer 11

When venipuncture is contraindicated

eg. Babies, small children, burn victims

Question 12

When Drawing pediatric blood specimen the order of draw is different in what way?

Answer 12

Reverse order as compared to adults

Question 13

What is the order of Processing of blood specimen tube w/a sample?

Answer 13

Collect–> Invert–> Wait–> Centrifuge–> Analyze

Question 14

A Plain Red top Vacutainer tube Contains what, Yields what, and is used by what testing area in the Lab?

Answer 14

Contains: 
-No Anticoagulant
-May have clot activator (thrombin)
Yields: Serum
Testing Area in Lab:
-Chemistry
-Serology

Question 15

Why Is a Serum separator Gel used in a Serum Separator Tube (SST)?

Answer 15

To separate blood cells from Serum to prevent further metabolic changes (i.e. glucose, clotting)

Question 16

What provides the barrier in a Serum Separator Tube (SST)?

Answer 16

Inert Polymer Barrier Gel

Question 17

A Red and Gray (SST), “Marble” top Vacutainer tube Contains what, Yields what, and is used by what testing area in the lab?

Answer 17

Contains: 
-No Anticoagulant
-May have clot activator
-Gel
Yields: Serum
Testing Area in Lab:
Chemistry

Question 18

A Lavender “Purple” top vacuainer tube Contains what, Yields what, and is used by what testing area in the lab?

Answer 18

Contains:
Anticoagulant- EDTA
Yields: Plasma
Testing Area:
Hematology
Blood Bank Testing

Question 19

An Un-Centrifuged Lavender tube can be used for what?

Answer 19

Blood Parasites (e.g. Malaria)