Exam 1 Flashcards
This Giemsa Stain shows the intracellular yeast form of what fungi?

Histoplasma capsulatum
What is the larger black round structure being pointed at?

Sporangium
What are the smaller circular structures being pointed at by two arrows?

spores
What are the elongated thin structures being pointed at?

hyphae
What dimorphic fungi takes these two forms?

Blastomyces dermatiditis
What dimorphic fungi takes these two forms?

Coccidiodes immitis
What dimorphic fungi takes these two forms?

Histoplasma capsulatum
What dimorphic fungi takes these two forms?

Paracoccidiodes brasiliens
Name this opportunisitic fungi

Aspergillus umigatus
Name this opportunistic fungi (with a large capsule)

Cryptococcus neoformans
Candida albicans is an opportunisitc fungi. What forms can it take?
Budding yeast, pseudohyphae, germ tube and hypha

What are the sites of action of antifungal drugs?
Inhibition of protein synthesis, inhibition of nucleic acid synthesis, disruption of microtubules and mitosis, direct membrane damage, ergosterol inhibition (cell membrane) and cell wall inhibitors of glucan or chitin

What special stain is used for this image of Rhizopus?

Silver stain
This fungus acts as a parasite to arthropods and nearly destroyed the silk industry in China

beauveria bassiana
Give four examples of dimorphic fungi
Coccidioides immitis
Sporothrix schenckii
Histoplasma spp.
Blastomyces dermatitidis
These are hyphae that are pigmented

Dematiaceous
Name for hyphae that are non pigmented

hyaline
Name these type of hyphae

coencytic
Name these type of hyphae

septate
Name this dimorphic fungi

Blastomyces dermatitidis
What are the two main types of asexual spores of fungi?
Sporangiospores and conidia
Name this asexual spore of fungi. What species is this common in?

sporangiospore
zygomycetes (Rhizopus)
Name this asexual form of fungal spores

conidia
Name this subtype of conidia (asexual spore). What species are common for A and B types?

Arthroconidia
A - dermatophytes B - Coccidioides immitis
Name this subtype of conidia (asexual spores). What species are all three types possible in?

Blastoconidia
Candida albicans
Name this subtype of conidia (asexual spores) that are thick walled and resistant. When would these be produced?

Chlamydoconidia
In a hostile environment
Name these large muti-celled conidia produced by dermatophytes in culture

macroconidia
Name these small conidia which are produced by certain dermatophytes

microconidia
Conidia which are produced from phialides are called what? What species are they common in?
Phialoconidia
Aspergillus

What are the three types of sexual spores for fungi? How are they formed?
Zygospores
Basidiospores
Ascospores
Formed from Meiosis
What’s another word for Gram Pos bacteria?
Firmacutes
What’s another name for Gram Neg bacteria?
Gracilicutes
What are mollicutes? What makes them special?
Mycoplasma; they lack a cell wall
Name two small obligate intracellular bacteria
Rickettsia and Chlamidia
Bacillus anthracis capsule is composed of what?
poly – D – glutamate
True or false: a true bacterial capsule is part of a cell wall?
False. It is outside the cell wall
what is a unique peptidoglycan found in bacterial cell walls? Do Archae have it?
Murein. No
Techoic acids are part of what type of bacterial component?
Cell walls of G+
What bacteria have an outer membrane composed of LPS (endotoxin)
G-
What are the two components of the LPS in G- bacteria and what do they do?
Lipid A - toxic portion; Polysaccharide O - antigenic, adherance, resistance
A dormant, tough, non reproductive structure in bacteria is called
an endosome
What part of the bacteria is composed of phospholipid with no sterols
Cytoplasmic membrane
What is dipicolonic acid and where is it found?
helps with resistance, found in endospores, not in vegetative cells
What are the two types of Superficial or cutaneous mycoses
Dermatophytoses (Microsporum and Trichophyton spp.);
Dermatomycoses (Malassezia, Geotrichum candidum and Trichosporon beigelii).
what are the two types of Sub-cutaneous mycoses
Sporotrichosis (Sporothrix schenckii); Epizootic Lymphangitis (H. capsulatum var. farciminosum)
What are the three types of systemic mycosis?
Blastomycosis (Blastomyces dermatitidis); Coccidioidomycosis (Coccidioides immitis);
Histoplasmosis (Histoplasma capsulatum var. capsulatum).
Tissue invasion by a fungus is called what?
mycosis
Toxin production by a fungus is called what?
mycotoxicosis
What would you collect occular fluid to diagnose?
ocular blastomycosis
For what diseases would you use a hematological stain to diagnose?
Sporotrichosis Histoplasmosis Pneumocytosis
For what diseases would you use a wet mount to diagnose?
Dermatophytosis (LPCB or KOH ), Mycetoma Phaeohyphomycosis Blastomycosis Coccidioidomycosis Aspergillosis Candidiasis (and other yeasts), Rhinosporidiosis Zygomycetes Protothecosis
For what disease would you use india ink to diagnose?
Cryptococcosis
Identify this dermatophyte. What animal(s) is it common in?

M. canis (6-12 cells). Most common dermatophyte of all animals. Common in cats.
Identify this dermatophyte. What animal(s) is it common in?

M. Gypseum (less than 5 cells); dogs and equine
Identify this dermatophyte. What animal(s) is it common in?

M. nanum (2 cells, bubbles); swine
Identify this dermatophyte. What animal(s) is it common in?

T. mentagrophytes ; dogs
Identify this dermatophyte. What animal(s) is it common in?

T. verrucosum; cattle (the only one to have a vaccine)
Name the causative agent for this disease in dogs.

M. canis, M. gypseum, T. erinacei, T. mentagrophytes
This lesion is a more severe form of what? Name the lesion. What is it caused by?

ringworm in dogs. Infection by T. mentagrophytes leads to intense inflammation (kerion)
Name the causative agent for this disease in cats. Can this cause any other problems in cats?

M. canis; feline otitis
Name the causative agent for this disease in cattle.

Trichophyton verrucosum
Name the causative agent for this disease in swine.

Microsporum nanum, followed by M. gypseum, M. canis and T. mentagrophytes.
Name the causative agent for this disease in horses.

Trichophyton equinum and M. gypseum
Name the causative agent for ringworm in poultry
M. gallinae
What is the major infective form for ringworm?
Spores are the major infectious form
They are shed by the infected animal into the environment and may remain viable for months to years
What is this?

Dermatophyte Test Medium; Growth with red slant before 10 days indicates a dermatophyte
cutaneous infections resembling dermatophytoses are caused by _____ and called _____
Yeasts and normally saprophytic filamentous fungi dermatamycoses
What are three examples of dermatamycoses
Malassezia
Trichosporon spp.
Geotrichum candidum
True or false: M. pachydermatis is part of the normal flora of animals?
TRUE
In dogs, M. pachydermatis causes
– Otitis externa
– Seborrheic dermatitis
What is this disease in a dog? What are the likely causative agents?

Otitis externa; Malasezzia pachydermatitits, Staph. Pseudointermedius, enterococcus
You made this slide from a swabbed ear of a dog suffering from otitis externa. What is your Dx?

Malassezia pachydermitits; shoe-print shapes
You obtained these slides from samples of a dog suffering from dermatitits. What is your dx?

Malesezzia pachydermitits
You obtained this sample from the skin of a reptile. What do you suspect?

Dermatomycoses caused by Geotrichum candidum
A cat presents with chronic, ulcerative lymphangitis of skin and subcutis. What do you suspect?

Sporothrix schenckii
This sample was obtained from exudate from a cat with Sporothrix Schenkii. Why would you not suspect this to be from any other animal?

other animals do not have abundant yeast in their exudates
What is this disease? What is it caused by

African Horse Farcy; Histoplasma capsulatum var. farciminosum
What is this and what is it caused by?

[Oomycosis] cutaneous pythiosis caused by Pythium insidiosum
What is this and what is it caused by? Is there anything special about this disease in horses?

[Oomycosis] cutaneous pythiosis caused by Pythium insidiosum; causes Kunkers or Leeches
What is this and what is it caused by

[Oomycosis] cutaneous pythiosis caused by Pythium insidiosum
What is this?

Chromoblastomycosis
What is this?

Phaeohyphomycosis
What is this?

Curvalaria
You obtained this slide from an aspirate of a lesion on a cat. What do you suspect?

Cryptococcus neoformans
(yeast organisms surrounded by a nonstaining capsule)
What is the likely causative agent?

candida albicans
Examination of exudates produced this slide. What do you suspect?

candida albicans
Shown is pseudohyphae. What is this from?

Candida albicans
This change in the canine scapula is caused by dissemination of what agent?

Coccididiodes immitis
This granulomatous inflammation of a horse’s lung was likely caused by:

Coccidiodes immitis
Skin scrapings of a cutaneous lesion contains this. What do you suspect?

Coccidioides immitis (endosporulating spherules)
Shown are the dimorphic stages of

Histoplasma capsulatum var. capsulatum
Shown are two examples of intracellular forms of this:

Histoplasma capsulatum
The non-normal lung is possibly caused by

histoplasmosis
Shown are the dimorphic forms of this fungus which affects humans, dogs (and rarely horses and cats)

Blastomyces dermatitidis
Shown is the histology slide from aspirate taken from a lesion in a horse’s nose. What is the likely agent?

Rhinosporidium seeberi
This causes a furry white appearance on the skin of dolphins

Lacazia loboi
This lesion on a goat was caused by what?

Tick pyemia – tick bite allowed Staph aureus normal flora to enter the body. The tick also carries Anaplasmaphagocytophilum which is an immunosuppressor
“greasy pig” disease is caused by what? What is the key virulence factor?

Staph Hyicus; Exfoliative toxin
The causative agent for “bumble foot” in birds is

Staph Aureus
The Lancefield Serological grouping can be used for what genus of bacteria?
Streptococcus
This causes strangles in horses. What is another name for stranges?
Strep equi subsp. equi
Highly contagious febrile rhinopharyngi*s
This suppurative osteomyelitis in a foal was caused by a Naval Ill infection induced by what?

Streptococcus zooepidemicus
The diplococcis observed here from collected from the luminal gastric mucous of a pig with jowl abscess (Cervical lymphadenitis)

Strep porcinus
Porcine syringomyelitis and porcine valvular endocarditis may both be caused by

Strep suis
The most common cause for pectoral abscess in a horse is

cornyebacterium
Caseous lymphadenitis in sheep is common caused by what?

cornyebacterium pseudotuberculosis
corynebacterium pseudotuberculosis has a synergistic effect with
rhodococcus equi
cornyebacterium pseudotuberculosis has an antagonistic effect on
staphylococcal beta toxin
Ovine Pisthosis is commonly caused by

corynebacterium renale
strep agalactiae have a synergistic effect on
Staphlococcal Beta toxin (CAMP test)