Exam 1 Flashcards

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1
Q

What would be the most obvious outcome of repeated cell cycles consisting of S
phase and M phase only?

A

The cells produced would get smaller and smaller

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2
Q

Which of the following events does not usually occur during interphase?

A

The nuclear envelope breaks down

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3
Q

Which of the following descriptions is consistent with the behavior of a cell that
lacks a protein required for a checkpoint mechanism that operates in G2?

A

The cell would enter m phase under conditions when normal cells would
not

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4
Q

Levels of CDK activity change during the cell cycle, in part, because

A

Cyclin levels change during the cycle

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5
Q

The concentration of mitotic cyclin (M cyclin)

A

Falls towards the end of M phase as a result of ubiquitylation and
degradation

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6
Q

Which of the following does not occur during M phase in animal cells?

A

Growth of the cell

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7
Q

The principal microtubule organizing center in animal cells is the

A

Centrosome

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8
Q

The classic experiments conducted by Meselson and Stahl demonstrated that
DNA replication is accomplished by employing a ____ mechanism.

A

Semiconservative

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9
Q

You have a piece of a circular DNA that can be cut by the restriction nucleases
Xhol and Smal, as indicated by the figure. If you were to cut this particular piece
of DNA with both Xhol and Smal, how many fragments of DNA would you end up
with?

A

2

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10
Q

During gel electrophoresis, DNA fragments

A

Migrates towards a positively charged electrode

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11
Q

A double stranded DNA molecule can be separated into single strands by
heating it to 90°C because

A

Heat disrupts hydrogen bonding between complementary nucleotides

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12
Q

You’re interested In a single stranded DNA molecule that contains the following
sequence. Which molecule can be used as a probe that will hybridize to your
sequence of interest? (5’…GATTGCAT…3’)

A

5’-ATGCAATC-3’

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13
Q

As two strands of DNA are unraveled, which enzyme relieves the strain on the
two strands?

A

Dna gyrase

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14
Q

DNA replication always proceeds by adding new bases to the ____ end
of an existing strand

A

3’

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15
Q

Which of the following molecules are not found in plants?

A

Collagen

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16
Q

Tight junctions

A

Are formed from claudins and occludins

17
Q

At desmosomes, cadheren molecules are connected to

A

Intermediate filaments

18
Q

Which of the following statements about gap junctions is true?

A

Gap junctions are made of connexons

19
Q

Which type of junction involves a connection to the actin cytoskeleton?

A

Adherens junctions

20
Q

Which type of junction contributes to the most to the polarization of
epithelial cells?

A

Tight junctions

21
Q

Plasmodesmata

A

Permits small molecules to pass from one cell to another in plants

22
Q

A malignant tumor is more dangerous than a benign tumor because

A

Its cells invade other tissues

23
Q

Hershey and Chase used radioactive phosphorus and sulfur to

A

Differentially label proteins and DNA

24
Q

The difference in leading versus lagging strand synthesis is a consequence of

A

Both the physical structure of DNA and the action of polymerase enzyme

25
Q

If the activity of DNA ligase was removed from replication, this would have a
greater effect on

A

Synthesis of the lagging strand versus the leading strand

26
Q

DNA gyrase

A

string of each DNA strand when supercoiling

27
Q

DNA polymerase III

A

no leading / lagging strand and no DNA synthesis

28
Q

Ligase

A

fragments will not connect

29
Q

DNA polymerase 1

A

primer stays/ unable to build

30
Q

Get one main difference between prokaryotic and eukaryotic replication

A

Eukaryotes have many origins of replication, prokaryotes only have one

31
Q

How long are the repeats in telomeres in bacteria

A

Zero / none