Exam 1 Flashcards
What would be the most obvious outcome of repeated cell cycles consisting of S
phase and M phase only?
The cells produced would get smaller and smaller
Which of the following events does not usually occur during interphase?
The nuclear envelope breaks down
Which of the following descriptions is consistent with the behavior of a cell that
lacks a protein required for a checkpoint mechanism that operates in G2?
The cell would enter m phase under conditions when normal cells would
not
Levels of CDK activity change during the cell cycle, in part, because
Cyclin levels change during the cycle
The concentration of mitotic cyclin (M cyclin)
Falls towards the end of M phase as a result of ubiquitylation and
degradation
Which of the following does not occur during M phase in animal cells?
Growth of the cell
The principal microtubule organizing center in animal cells is the
Centrosome
The classic experiments conducted by Meselson and Stahl demonstrated that
DNA replication is accomplished by employing a ____ mechanism.
Semiconservative
You have a piece of a circular DNA that can be cut by the restriction nucleases
Xhol and Smal, as indicated by the figure. If you were to cut this particular piece
of DNA with both Xhol and Smal, how many fragments of DNA would you end up
with?
2
During gel electrophoresis, DNA fragments
Migrates towards a positively charged electrode
A double stranded DNA molecule can be separated into single strands by
heating it to 90°C because
Heat disrupts hydrogen bonding between complementary nucleotides
You’re interested In a single stranded DNA molecule that contains the following
sequence. Which molecule can be used as a probe that will hybridize to your
sequence of interest? (5’…GATTGCAT…3’)
5’-ATGCAATC-3’
As two strands of DNA are unraveled, which enzyme relieves the strain on the
two strands?
Dna gyrase
DNA replication always proceeds by adding new bases to the ____ end
of an existing strand
3’
Which of the following molecules are not found in plants?
Collagen