Exam 1 Flashcards
pipettors or micropipettors
mechanical pipettes used to measure volumes ranging from .01 microliters to 5000 microliters
what do pipettors have
plunger with 2 stops, a barrel, an extension that holds disposable tip, a pipette tip ejector
some have a filter
what do you change between different solutions or fluids
pipette tip
adjustable pipettor
has a dial or digital display that allows you to set the desired volume
where is the maximum capacity of an adjustable pipettor typically inidicated
on top of the plunger
what is the dial of a pipettor marked in
microliter increments and the position of the decimal point is usually indicated by a colored line
if a pipettor is used outside of their range what happens to their accuracy
the accuracy is greatly reduced
can you go over the maximum volume of a pipettor or below the minimum
no, never go past these
can the tip of pipettor touch anything
no, nothing but the solution, start with a new tip
how to work a pipettor
push plunger to first stop, then slowly release to pull in liquid, push past first stop to second stop to push out the liquid
to remove the tip, push tip ejector
what happens if you release the plunger too fast
you can get air in the tip
should you leave the plunger depressed when removing after releasing fluid
yes
how far should you put the tip in a fluid
about 3mm
where should you eject a tip
in an appropriate waste container
serial dilation
transfer of concentrated stock solution across several containers
what is serial dilution used for
a quick and easy way to produce a solution in a variety of concentrations
can also be the only way to effectively and accurately dilute an extremely concentrated solution
what is the difference between each of the various concentrations that result from a serial dilution?
constant
how does the difference in serial dilations work
each one is one half the concentration of the next highest, or each one is one tenth the concentration of the next highest
how to do serial dilution
have 2000 microliters of solution and 5 tubes with 1000 microliters of water
take 1000 from solution and put in tube 2 and mix, continue down the tubes to tube 6
what are ELISA plates used for
for enzyme linked immunosorbant essays
what is an ELISA used to measure
used to measure antibody and hormone levels
what objective should you start with on your microscope
the lowest objective
where should your stage be when starting with microscope
high
should you turn on the light on your microscope and adjust brightness if needed
yes