Ex 3:Aseptic Transfer Techniques

Question 1

What are aseptic/sterile techniques?

Answer 1

procedures done to protect the culture, worker, and environment from contamination

Question 2

What is a pure culture?

Answer 2

culture free of contamination and containing organisms descended from one original cell

Question 3

Why are pure cultures important?

Answer 3

for identifying bacteria with biochemical tests, performing antibiotic sensitivity testing or maintaining stock cultures

Question 4

What is a pathogen?

Answer 4

disease producing micro-organism

Question 5

Why do microbiologists need to transfer bacteria?

Answer 5

bacteria need fresh nutrients, be removed from their own toxic waste products, or when biochemical testing of an isolated species is necessary

Question 6

What is flaming?

Answer 6

sterilizing loops/needles by holding wire into a flame until red hot

Question 7

What are aerosols?

Answer 7

droplets of micro-organisms

Question 8

What is a bacticinerator?

Answer 8

special oven that sterilizes needles/loops, culture tubes. consists of a ceramic funnel tube

Question 9

What is a pipet used for?

Answer 9

transfer of liquids including sterile culture mediums, bacterial culture or reagents

Question 10

What is alcohol flaming?

Answer 10

dangerous sterilization technique that sterilizes forceps by dipping them into alcohol and passing through a flame