EUKARYOTIC TRANSCRIPTION

Question 1

Where does eukaryotic transcription take place?

Answer 1

Nucleus

Question 2

What is needed for transcription to begin?

Answer 2

Transcription factors

Question 3

What are the transcription factors required to bind to?

Answer 3

TATA box promoter

Question 4

What are the 3 overall steps?

Answer 4

Initiation, elongation, termination

Question 5

Does transcription and translation happen simultaneously?

Answer 5

No

Question 6

What changes happen to the mRNA after transcription?

Answer 6

Splicing - introns removed, exons spliced together
Cap added to 5 and and poly A tail added to 3 end of the sequence

Question 7

When does termination occur?

Answer 7

Once the polymerase has transcribed the DNA

Question 8

How is eukaryotic transcription regulated?

Answer 8

• histone acetlylation
• transcription factors
• enhancers (change shape of DNA)
• repressors

Question 9

What happens in eukaryotes pre mRNA?

Answer 9

Introns are removed and exons come together - splicing

Question 10

What does mRNA code for?

Answer 10

Proteins

Question 11

What does rRNA code for?

Answer 11

Structure and function of ribosomes

Question 12

What does tRNA code for?

Answer 12

Delivers amino acids for protein synthesis

Question 13

What RNA pol initiates transcription?

Answer 13

RNA pol II