Eukaryotic Transcription

Question 1

Where is TATA Box?

Answer 1

At -35 region

Question 2

CAT Box

Answer 2

At -75 region

Question 3

Transcription Initiation Site

Answer 3

+1

Question 4

Basal Transcription Factors

Answer 4

Regulates initiation of transcription from any/all promoter(s)
- Binds to promoter sequence and allows for RNA pol II to bind

Question 5

Regulatory Transcription Factors

Answer 5

Regulates initiation of transcription from a small number of promoters
- Binds either after promoter sequence or a difference sequence

Question 6

RNA Polymerase II

Answer 6

Transcribes protein coding genes

Question 7

Cis-Acting Elements

Answer 7

HAVE to be on the same DNA molecule as promoter

Question 8

Trans-Acting Elements

Answer 8

Does NOT have to be on the same DNA molecule as promoter

Question 9

Carboxy Terminal Domain (CTD)

Answer 9

The carboxy terminus of polypeptide

Question 10

Phosphorylation

Answer 10

Adding a phosphate on a protein

Question 11

Capping

Answer 11

• Addition of a 5’ methyl G cap to 5’ end
• 5’—-5’ linkage (weird)
• stabilizes mRNA

Question 12

Splicing

Answer 12

Introns are removed from primary transcript and exons are joined together

Question 13

Introns

Answer 13

INtervening

Why you take them out

Question 14

Exons

Answer 14

EXpressed

Question 15

snRPS

Answer 15

• “Small Nuclear Ribonucleoproteins”
• Recognizes intron ends and forms a spliceosome

Question 16

Ribozymes

Answer 16

Catalyzes process of snRPS and reactions of different genes

Question 17

Isoforms

Answer 17

Different forms of a product from the same gene
- Leads to different forms of proteins
- Allows for more regulation of process

Why introns are important!

Question 18

Exon Shuffling

Answer 18

2 or more exons from different genes brought together

Question 19

Poly-A Adenylace

Answer 19

• Makes RNA molecule more stable
• Prevents degredation

Question 20

Self-Splicing Reaction

Answer 20

Does NOT involve proteins

Question 21

Trans-Splicing Function

Answer 21

Combining exons from different genes

Question 22

Termination

Answer 22

• Involves cleavage at the signal sequence and the addition of a poly-A tail
• Cleavage done by endonuclease

Question 23

Domain

Answer 23

Region of a protein with specialized functions