eukaryotic RNA polymerase and promoters Flashcards
what does RNA polymerase 1 do?
what is its sensitivity to alpha amanitin?
how many subunits does it have?
transcribes rRNA genes
insensitive to alpha amanitin
14 subunits
what does RNA polymerase 2 do?
what is its sensitivity to alpha amanitin?
how many subunits does it have?
transcribes mRN
highly sensitive to alpha amanitin
12 subunits
what does RNA polymerase 3 do?
what is its sensitivity to alpha amanitin?
how many subunits does it have?
transcribes small RNAs
moderately sensitive to alpha amanitin
17 subunits
which eukaryotic polymerases are responsible for 80% of a cells transcription?
1 and 3
what does RNA polymerase 4 and 5 do?
ranscribe regulatory RNAs in plants
what is Rpb1?
the subunit that gets modified during the elongation phase of transcription
what is the most profound different between bacterial and eukaryotic polymerase?
the stalk
what are eukaryotic promoters?
core elements which general transcription factors bind to position the RNA polymerase.
where is the TATA box located and what is it recognized by?
25-30 base pairs upstream - recognized by a subunit of TFIID (TATA box binding protein/TBP)
waht is BRE?
binding site for TFIIB
what are general transcription factors?
required for all or many genes, found in all cells at all times (Housekeeping)
what are specific transcription factors?
activate/inactivate only one or a few genes and are found in a tissue specific or time specific fashion
where is the CAAT box located?
-80
where is the GC rich box loacted?
-90
what are HeLa cells?
Derived from immortalized cervical cancer cells
Henrietta Lacks
