Essential controls and reproducibility in flow cytometry

Question 1

What are the “essential controls”? There are seven of them

Answer 1

FMOs, Gating controls, Isotype controls (supersede by FMOs), Viability Stains, Unstained, Single Stain, Instrument maintenance

Question 2

How to make flow papers reproducible? four ideas with graphing having three subsections

Answer 2

Following the guidelines of OMIP, include filter and band width, (when graphing have sequential gating, color/marker axis, and % of populations)

Question 3

Name some examples that harms reproducibility

Answer 3

selective reporting, pressure to publish, low statistical power or poor analysis, insufficient #of replication and oversight of work, lack of information on the methodology, poor experimental design, lack of availability of original data, fraud, insufficient peer review, lack of technical expertise, variability of standard reagents, and bad luck.