Essays Flashcards
1
Q
Biomaterial experiments
A
- EBSC retinal pigment epithelium- spit fighter planes
- Bioglass- rat implant bone
- Mechanical stimuli on stiffness
- Cell:material interaction- cell shape controls cell fate- micropatterning of fibromectin into island- same GF,ECM but different geometry= different fates
2
Q
Scaffold experiments
A
- 3D print human tissue- 15x15x1 myoblast- PCL pillar, thrombin solution for fibronectin gelatine and uncross links
- Bone generation- BMP entrapped in PEG- crosslinks added site for cleavage and BMP liberated which activates osteoblasts- bone matrix
3
Q
Bioreactors
A
- Cell seeding distribution- bone marrow stromal cells, MTT look at seeding in perfused and static
Chondroctyes seeded and only mixed survived - Porcine heart- decellularising
- Mauck et al- articular cartilage- loading pressure
- Tammy et al- space flight conditions on osteoclasts
- TE heart valves flex stretch flow system- increase function of heart valve
- Pulsatile pump blood vessels
- Vocal folds
4
Q
Skin substitutes
A
Epidermal keratinocytes layers
- My skin- subconfluent autologous keratinocytes, diabetic foot ulcer
- Composite substitutes- using allogenic skin incorporated into the scaffold
- apligraf- bovine 1 collagen, fibroblasts keratinocytes- normal skin
- OrCell- seeded fibroblast and keratinocytes onto collagen sponge
5
Q
Gene therapy
A
- EB boy- splice site mutation in 14 intron of lambB, epidermal transplant with corrected layer
- Make 3D skin- PSC CDB approach- 0-30 days
- Hair follicle- from male- incutaneous transplant and orthoptic hair follicle in nude female
6
Q
Nerve regeneration
A
- Matrigel- protein from mouse sarcoma- promote regeneration from ecm protein
- Medial nerve , nerve conduit- close gap
7
Q
Vascularization
A
- Folkman et al- Tumour angiogenesis, cut vasculature from tumour and it shrinks
- Polymeric system dual GF delivery- VEGF and PDGF form mature vasculature network
8
Q
Cornea
A
- Limbal study- long term restoration to damaged cornea- holoclur - 74/104 worked no surface problems, reduced pain, restored sight
- cornea alternative cells- oral mucosa
9
Q
Host response
A
- Diabetes type 1 insulin deficiency- cadaver islet cell transplantation
- Immunoisolation- semi permeable, protect insulin
10
Q
Organ on a chip
A
- Drug withdrawn from market- VIOXX hepatotoxic, cardiotoxic
- PGN14N12- immmunotherapy for leukaemia- thought revolutionary but killed 6 patients in phase 1 due to difference in aa of primates and humans
- Pulmonary inflammation- TNFa activated- release iCAM (measured)- leukocyte to capillary alveolar barrier- ecoli GFP
- Pulmonary odema- cancer drugs IL-2, add to vascular side, permeability decreases and water fills chamber
11
Q
Regulation
A
1c GO GO TE wen bankrupt, dermal pigment, 40 patients fundraised for skim substitutes
2. Burn victims, FDA approved but delays meant it never happened
