Equations

Question 1

Resolution

Answer 1

0.61λ/NA

Question 2

Henderson-Hasslebach

Answer 2

pH=pKa+log (base/acid)

Question 3

Michaelis-Menten

Answer 3

v0=vmax[S]/([S]+Km]

Question 4

NA

Answer 4

n*sinѲ
n=the refractive index of the material that the light travels through from the subject to the lens
Ѳ=1/2 the angle that light enters the lens

Question 5

absorbance

Answer 5

2-log%T or log(1/T)

Question 6

Beer-Lambert Law

Answer 6

A=elc

Question 7

DNA in ug/mL

Answer 7

Abs x dilution factor x 50

Question 8

Kcat

Answer 8

Vmax/[E]

Question 9

Activity

Answer 9

(C/min)(reaction volume)/enzyme volume

Question 10

Specific activity

Answer 10

Activity/[protein]

Question 11

Likelihood of a cut site?

Answer 11

4^N, where N-length of cut site in bp

Question 12

of copies in PCR

Answer 12

(2^n-2n)x where n=number of cycles and x=numbers of copies we started with

Question 13

Tm

Answer 13

2C(A+T)+4C(G+C)
more complicated hwoever to calculate Tm if you take into account order of bases and salt conc OR
64.9C+41Cx(G+C-16.4)/N

Question 14

Partition coefficient

Answer 14

Kav=(Ve-Vo)/(Vt-Vo)