Epigenetics Flashcards
What is chromatin?
Complex of DNA and proteins into which eukaryotic chromosomes are packaged
What is included in a nucleosome?
An octomeric histone core with 2 subunits of H2A, H2B, H3, and H4, with 147 base pairs of DNA
What are histones?
Positively charged DNA binding proteins with flexible tails
What is linker DNA?
DNA between histones
What do H1 histones do?
Linker histones that are involved in the assembly of nucleosomes into higher order structures
What are the two types of chromatin?
Euchromatin and heterochromatin
What is heterochromatin? Is it being transcribed?
Chromatin that is highly dense and compacted. It is not being transcribed
What is euchromatin? Is it being transcribed?
Chromatin that is more loosely packaged. Is being transcribed
Is chromatin always either euchromatin or heterochromatin?
No, it’s a lot more complicated and chromatin can quickly shift between the two
What are 3 common histone modifications?
Methylation, acetylation, and phosphorylation
What is histone acetylation?
Acetyl groups are added to lysine residues in the histone tails. They are rapidly reversible
What type of enzyme adds acetyl groups to histones?
Histone acetylases (HATs)
What type of enzyme removes acetyl groups from histones?
Histone deacetylases (HDACs)
Does histone acetylation typically activate or repress gene expression?
Activate
Does histone methylation typically activate or repress gene expression?
Can be either