enzymes - the effect of pH on enzyme activity

Question 1

what does the pH indicate ?

Answer 1

whether a substance is acidic , alkaline or neutral

Question 2

what is the pH values of acidic ?

Answer 2

0-6

Question 3

what is pH values of neutral ?

Answer 3

7

Question 4

what is the pH values of alkaline ?

Answer 4

8-14

Question 5

what acids dissociate and what itno ?

Answer 5

hydrochloric acids + sulfuric acid
–> protons and negatively charged ion

Question 6

what is the reaction for hydrochloric acid ?

Answer 6

HCl –> H+ + Cl-

Question 7

what is the reaction for sulfuric acid ?

Answer 7

H2SO4 –> H+ _ HSO4-

Question 8

what organic acids are ?

Answer 8

proton donors

Question 9

what are the organic acids and what they dissociate into ?

Answer 9

lactic acids - dissociates into H+ and lactate
pyruvic acids - dissociates into H+ and pyruvate

Question 10

what is a buffer in biology ?

Answer 10

something that resists change in pH

Question 11

what do some certain chemicals in your blood do and why?

Answer 11

help resist changes in pH
–> so that blood remains within fairly narrow limits close to pH 7.4

Question 12

what can these chemicals do ?

Answer 12

donate and accept hydrogen ions

Question 13

what can haemoglobin do and act similar to ?

Answer 13

can also donate or accept protions
- act as buffers

Question 14

what would buffer solution be used for ?

Answer 14

to maintain the desired pH for investigating enzymes action at different pH values
OR
to keep the pH constant whilst you investigate another factor

Question 15

what is the pH worked out by - formula ?

Answer 15

• 1/(H+)

Question 16

what is the proportion / relationship between conc and pH ?

Answer 16

a large concentration of hydrogen ions gives a small value of pH
–> if the acidity increases then the pH decreases

Question 17

what are hydrogen ions ?

Answer 17

protons