Enzymes practical

Question 1

1

Answer 1

Put a drop of iodine solution into every well of a spotting tile.

Place a Bunsen burner on a heat proof mat, a tripod and a gauze over the Bunsen burner. Put a beaker of water and heat it up to 35 degrees

Question 2

2

Answer 2

Use a syringe to add 3cm^3 of amylase solution and 1cm^3 of a buffer solution with a PH of 5 to a boiling tube

Question 3

3

Answer 3

Different syringe to add 3cm^3 of a starch solution to the boiling tube.

Question 4

4

Answer 4

Mix the contents of the boiling tube and start the stopwatch

Question 5

5

Answer 5

Do continuous samplings to record how long it takes for the amylase to breakdown.

Question 6

6

Answer 6

Repeat using different PH values to see how PH affects the time that starch breaks down

Question 7

Controlled variables

Answer 7

Concentration of amylase solution
Volume of amylase solution

To make it a fair test

Question 8

State the hazard and safety precaution

Answer 8

Iodine solution is an irritant. Avoid contact with skin or eyes