Enzymes Part 2 Flashcards
What are the 6 classes of enzymes and their functions
Oxidoreductases - REDOX transfer electrons
transferases - transfer functional groups
hydrolases- Hydrolysis
lyases- Make double bonds
Isomerases- isomerism internal transfers
Ligases- join things together - covalent bond
Example of oxidoreductases
Cytochromes
Example of transferases
kinases
example of hydroases
proteases
example of lyases
dehydratases
example of isomerases
topoisomerases
example of ligases
DNA ligases
What is P450s
Cytochromes
What are P450s used for
Detoxification of unwanted molecules from the body
What is the active site of P450s
Heme group
What does kinases do
Transfer a phosphate group from ATP to another protein
Issue of over-active kinases
Leads to cancer
What’s kinases importance
signalling immune response
Where are kinases found
Often attached to receptors through the cell membrane
Function of proteases
Break peptide bonds
What’s the 2 classification of proteases depend on
Base on catalytic site
base on pH in active
What happens if someone is dehydratase deficient ?
hyperphenylalaninemia which is over presence of phenylalanine in blood
What’s the function of Topoisomerases?
Take knots out of DNA
Function of DNA ligase
Repair breaks in DNA by using ATP in order to make new phosphate bonds in DNA backbone
What’s DNA ligase used in in molecular biology
PCR
Define enzyme Regulation
The process by which the activity of an enzyme is modulated or controlled in order to maintaining optimal metabolic activity. The control of protein population
What are the 4 types of regulations
Genetic expression
compartmentalization
feedback control
degradation
