enzymes in DNA replication

Question 1

Helicase

Answer 1

uncoils the DNA double helix and splits it into two template strands
- breaks the hydrogen bonds between the bases

Question 2

RNA primase

Answer 2

adds a short length of RNA to the template strand of DNA

- this acts as a primer, allowing DNA polymerase to bind and begin replication
- very important in the lagging strand

Question 3

DNA Polymerase III

Answer 3

adds nucleotides in a 5’ to 3’ direction
- can also attach to the 3’ end of the original strand

starts replicating next to the RNA primer and adds nucleotides in a 5’ to 3’ direction

Question 4

DNA Polymerase I

Answer 4

removes the RNA primer and replaces it with DNA

Question 5

DNA Ligase

Answer 5

seals up the leading and lagging strands

Question 6

DNA Primase

Answer 6

Initiates the synthesis DNA by creating a short RNA segment at replication fork

Question 7

SSB Proteins

Answer 7

Stabilizes the DNA molecule during replication