Enzymes (biomoleq)

Question 1

Describe and explain the mode of enzymes in terms of active site, enzyme-substrate complex,
lowering of activation energy and enzyme specificity.

Question 2

Explain how enzymes lower the activation energy of a reaction.

Question 3

Distinguish between the lock-and-key hypothesis and induced-fit hypothesis of the mechanism
of enzyme action.

Question 4

Describe and explain how the rate of formation of products in an enzyme-catalysed reaction
(e.g. using catalase) may be measured.

Question 5

Describe and explain how the rate of change of substrates in an enzyme-catalysed reaction
(e.g. using amylase) may be measured.

Question 6

Describe and explain the effect of temperature on the rate of enzyme-catalysed reactions.

Question 7

Describe and explain the effect of pH on the rate of enzyme-catalysed reactions.

Question 8

Describe and explain the effect of enzyme concentration on the rate of enzyme-catalysed
reactions.

Question 9

Describe and explain the effect of substrate concentration on the rate of enzyme-catalysed
reactions.

Question 10

Explain the effects of competitive and non-competitive inhibitors on the rate of an enzyme-
catalysed reaction.

Question 11

Explain how feedback inhibition can be used to regulate metabolic pathways in a cell.