Enzymes and Coenzymes Flashcards
Induced fit model a.k.a
Daniel Koshland’s model
Significance of Km
Affinity of enzyme
Mechanism of enzyme inhibitors is described by this model
Lineweaver-burk plot
What type of inhibition?
Increased km, Vmax unchanged
Competitive Inhibition
Km unchanged, Vmax decreased
Noncompetitive inhibition
Competitive inhibitor of HMG-CA
Simvastatin
Transfer of Acyl groups and active aldehyde
TPP, Lipoic acid, Coenzyme A
“TLC”
Amino transfer
Pyridoxal Phosphate
“AMerican Py”
Activation and Transfer of Co2
Biocitin
“carbon DI oxide”
Transfer of carbon compounds
Tetrahydrofolate
Transfer of Alkyl groups
Cobamide (B12)
Synthessized form biopterin
Tetrahydrobiopterin
What enzyme catalyzes Phenylalanine hydoxylase, and Tryptophan Hydroxilaze
Tetrahydrobiopterin
Deficiency in this enzyme causes PKU
Tetrahydrobiopterin, (Phenyl hydroxylase)
What coenzyme is derived from B1
Thiamine Pyrophosphate
What ion is required for Thiamine pyrophosphate activity?
Mg+
3 enzymatic reactions that require TPP
Pyruvic acid decarboxylase
Transketolase
Pyruvate dehydrogenase
Responsible for oxidative decarboxylation of pyruvate acid
Pyruvate dehydrogenase
What inhibits biocytin synthesis?
Avidin
2 enzyme that requires biocytin
Acetyl COA carboxylase
Pyruvate carboxylase
Tetrahydrofolic acid consists:
Subsituted pteridine
P-Aminobenzoic acid (PABA)
glutamic acid
Responsible for one-carbon group transfer except CO2
Tetrahydrofolic acid
Tetrahydrofolic acid inhibitor
Methotrexate, sulfonamides
Cobamide is derived from?
Vit B12 or Cyanocobalamin
