Enzymes Flashcards
1
Q
Lock and Key model
A
2
Q
Induced fit model
A
3
Q
Factors affecting enzyme function
A
- pH, salinity, temperature, cofactor association
- Apoenzyme: enzyme without its cofactor
- Holoenzyme: enzyme with its cofactor
4
Q
Enzyme Regulation
A
- covalent modification i.e. phosphorylation
- proteolytic cleavage (zymogens)
- Association with other proetins
- Allosteric regulation (feedback inhibition)
5
Q
Proteolytic cleavage
A
i.e. zymogens
6
Q
Saturation
A
All active sites are occupied by substrate, so increasing [s] will not increase the rate (Vmax)
7
Q
Km (michaelis constant)
A
- Substrate concentration at 1/2 Vmax
- The smaller the Km, the greater affinity for the substrate
8
Q
Michaelis-Menten equation
A
9
Q
Lineweaver-Burk Plots
A
10
Q
Competitive, uncompetitve, noncompetitive
inhibition
A
11
Q
Oxidoreductases
A
catalyze redox reactions (electron transfer)
12
Q
Transferases
A
catalyze transfer of functional groups
13
Q
Hydrolases
A
catalyze cleavage of molecules driven by the addition of water
14
Q
Lyases
A
catalyze addition of atoms or groups to a double bond
15
Q
Isomerases
A
catalyze transfer of functional groups within a
molecule (creates different isomers of the same molecule)
i.e. mutase
