Enzymes Flashcards
What is the transition state in a reaction?
The high energy intermediate that lies between substrate and product in a chemical reaction
What is the activation energy for a reaction? What does it determine?
The minimum energy the substrate must have to allow the reaction to occur
How likely a reaction is to occur
In which two ways can increase the rate of reaction?
Changing the;
Temperature
Concentration
How does increasing the temperature affect the rate of reaction?
Increases the rate —-> increased number of molecules with activation energy
How does an increased concentration of substrate increase the rate of reaction?
Increased chance of molecular collisions
What is an enzyme?
Biological catalysts that increase the rate of reaction by lowering the activation energy
Enzymes promote the formation of ……
The transition state
Do enzymes affect the reaction equilibrium?
No
Are enzymes changed after a reaction?
No they remain unchanged
What is the active site of an enzyme? What shape do they usually take?
Where the substrate binds and where the chemical reaction occurs
Clefts or crevices
Which two models exist to describe how substrates bind to an active site? Which one is more valid?
Lock and key model
Induced fit model
Induced fit hypothesis is considered to be more correct
Describe the induced fit hypothesis of substrate binding to active site.
The binding of a substrate induces changes in the conformation of an enzyme —> still has a similar complementary shape to the active site
What is V0?
The initial rate of reaction (at t=0)
A tangent to the start of an enzyme reaction curve will show what?
The fastest rate of reaction
What shape curve do enzyme rate of reaction curves usually show?
Rectangular hyperbola
