Enzymes Flashcards
1
Q
Calculation of pH from H+
A
pH = -log_10 [H+]
2
Q
Michaelis Menten equation
A
vo= (Vmax+[S])/(Km+[S])
3
Q
Meaning of kcat and Km for an enzyme
A
Km=1/2Vmax the [S] value at which velocity is half max
kcat - turnover number. indicates how fast the enzyme can work under conditions in which there is saturating amount of substrate. a strong enzyme will have a large kcat value.
4
Q
kcat/Km
A
enzymatic rate constant
5
Q
slope for line weaver burke
A
Km/Vmax
6
Q
Shape of vo versus [S] in Michaelis Menten
A
7
Q
vo versus [S] curves for allosteric enzymes
A
8
Q
Find Vmax and Km on L-B
A
y-int = 1/Vmax
x-int= -1/Km
