enzymes Flashcards

1
Q

biomechanical pathways

A

the chains of steps in photosynthesis and cellular respiration, in which the product of one reaction becomes the substrate in another
- represented by a series of letters and arrows

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2
Q

cells have ways of removing the final product so that biochemical pathways keep operating in the right direction.
-photosynthesis

A

the final product is glucose. it is converted to starch (insoluble) and stored. this allows the plant to keep producing and storing more glucose

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3
Q

cells have ways of removing the final product so that biochemical pathways keep operating in the right direction.
-cellular respiration

A

the products of the breakdown of glucose- CO2, h2O- diffuse out the cell, allowing the reactions to keep proceeding

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4
Q

metabolism

A

the range of biochemical process that occur in living organisms

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5
Q

cellular metabolism

A

the sum of all chemical reactions that take place in all living cells

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6
Q

the two types of cellular metabolism

A
  • anabolic
  • catabolic
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7
Q

endergonic

A

reactions requiring an initial input of energy to get started

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8
Q

exergonic

A

reactions NOT requiring initial energy input to get started

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9
Q

endothermic

A

reactions reaction that absorbs heat energy from its surroundings

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10
Q

exothermic

A

reactions that release heat energy (e.g. cellular respiration)

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11
Q

what reaction is cellular respiration

A

exothermic- energy is released in the form of heat

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12
Q

photosynthesis

A

the process where photoautotrophs use light energy to convert CO2 and h2O into glucose

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13
Q

where can photosynthesis occur

A

in chloroplasts of plant cells and some protists

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14
Q

why is photosynthesis an example of a biomechanical pathway

A

as it is a complex series of reactions, with each step within each reaction being catalysed by a specific enzyme of enzymes

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15
Q

photosynthesis pt 2- endo

A

photosynthesis is not endothermic reaction as although energy input if required (endergonic), it is not heat.

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16
Q

cellular respiration

A

the series of chemical reactions in which energy from organic compounds (glucose) is converted into an energy form that can be used by cells (ATP)

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17
Q

cellular respiration can be:

A

aerobic (in the presence of oxygen)
anaerobic (lacking oxygen)

18
Q

where does cellular respiration occur

A

cytosol and mitochondria of eukaryotic cells

19
Q

why is cellular respiration an example of a biochemical pathway

A

it is a complex series of reactions, with each step within each reaction being catalyzed by a specific enzyme of enzymes

20
Q

enzymes

A
  • a protein
  • biological catalysts to speed up chemical reactions in organisms - life would not exist without enzymes as reactions would occur too slowly (or not at all)
  • are usually specific to a specific reaction
21
Q

enzymes do not..

A

produce more product, they just speed up the reaction, which may produce more product in a given time period

21
Q

how do enzymes speed up the reaction

A

by reducing the activation energy (the amount of energy needed to begin the reaction)

22
Q

cofactors

A

some enzymes require additional molecules to enable them to catalyze a reaction
- inorganic molecules
- BINDS to the enzyme which enables the enzyme-catalyzed reaction to occur
- FRY PAN

23
Q

cofactors include:

A
  • Mg2+
  • Fe2+
  • Zn2+ ions
  • vitamins
24
coenzymes
- non-protein organic substances
25
coenzymes in photosynthesis and cellular respiration;
- coenzymes are reversibly loaded and unloaded with the groups of atoms they carry - they accept electrons and protons, and chemical groups (like phosphate) during reactions and transfer them to another reaction in a different step.
26
unloaded enzymes
unloaded enzymes accept electrons, protons or a chemical group. the coenzyme is now considered loaded.
27
enzymes are sensitive to:
- temperature - changing pH - this means the rate of photosynthesis and cellular respiration can be affected by changes in temperature and pH
28
the rate of photosynthesis and cellular respiration can also be affected by:
- enzyme concentration - substrate concentrations
29
temperature
most chemical reactions increase in speed as the temperature increases due to the greater speed and collision rate of molecules.
30
temperature- for enzyme catalyzed reactions
the enzyme denatures- bonds are broken and the protein changes shape, thus altering the shape of the active site, making the enzyme non-functional
31
at lower temps
molecules move slower and fewer collisions occur between the enzyme and substrate, resulting in lower rate of reaction. - in low temps the enzymes are inactive but not denatured
32
pH
each enzyme has an optimal pH - the pH at which it works best. - optimal pH of the enzyme varies depending on where it is
33
changes in pH
effects the amino acid and therefore the enzyme - if charges on the amino acid change, then the bonds maintaining the tertiary structure of the enzyme may be broken
34
enzyme and substrate concentrations
increases in enzyme concentration will increase the rate of reaction -there is usually a greater concentration of substrate than enzyme so any increase in enzyme should result in an increase in reaction rate
35
the increase in the rate only continues until...
the saturation point where all of the active sites become full
36
regulation of enzyme concentration
in response to the needs of the cell.
37
regulation occurs via
- controlling the expression of the enzyme - controlling the rate of enzyme degradation - activating the enzyme in response to a stimulus
38
enzyme inhibitors
- change the rate of reactions, either slowing them down or stopping them completely; - can be non-competitive or competitive
39
non competitive inhibitors
- do not bind to the active site. - bind to an alternative site called the allosteric site- causes the active site to change shape and no longer bind to the substrate
40
feedback inhibition
a way cellular respiration can be regulated through non-competitive inhibition
41
competitive inhibitors
directly compete with the substrate for the active site - the shape of the inhibitor is complementary to the shape of the active site.