Enzymes Flashcards
What enzyme is used to measure the reaction rate with starch?
Amylase
True or False: The pH level can affect the activity of amylase.
True
Fill in the blank: The substrate used in the reaction with amylase is __________.
starch
What is the primary product of starch hydrolysis by amylase?
Maltose
Which pH levels are typically tested for amylase activity?
Acidic, neutral, and alkaline pH levels
Multiple Choice: At which pH does amylase have the highest activity? A) 4 B) 7 C) 10
B) 7
What method can be used to measure the rate of reaction between amylase and starch?
Iodine test or spectrophotometry
True or False: Higher pH levels always increase the rate of reaction for amylase.
False
What factors can influence the rate of reaction between amylase and starch?
pH, temperature, enzyme concentration, and substrate concentration
Describe how to conduct a simple experiment to measure the rate of reaction between amylase and starch.
Mix amylase with starch at different pH levels, incubate, and measure the reaction time using iodine.