Enzymes Flashcards
What are globular proteins?
Polypeptide chains folded into a compact shape like a ball with an irregular surface
Complex tertiary and quaternary structure
What are fibrous proteins?
Individual molecules that span a large distance
Simple 3D structure
What is an enzyme?
A globular biological catalyst that speeds up metabolic reactions without being changed in the process
What are enzymes needed for?
Metabolism, movement, digestion, cell signalling and gene expression
What is a kinase enzyme?
These add phosphate ions to molecules
How are enzymes involved in disease?
May disrupt homeostasis e.g. PKU - rare genetic disorder caused by mutation leading to seizures and behavioural problems
What is the active site?
It is a cleft on the protein
It is non polar
Has specific amino acids that allow substrate to bind and others for catalysis
What is the induced fit model?
when substrates bind to the enzyme active site it will change its shape slightly so the chemical reaction can occur with the lowest energy possible
What is the allosteric site?
Away from the active site and is where regulatory molecules bind which can activate or inhibit a molecule
What is a co-factor?
Any inorganic factor required for enzyme activity e.g. metal ions for metalloenzymes
What is a coenzyme?
An organic molecule that is directly involved in enzyme catalysis e.g. lactate dehydrogenase
What is an apoenzyme?
Inactive - no coenzyme added
What is a holoenzyme?
Active - coenzyme added
What is a prosthetic group?
Non protein molecules that are associated with the enzyme via covalent bonds
What do oxidoreductases do?
Transfer oxygen or hydrogen atoms or electrons from one substrate to another