Enzymes Flashcards

1
Q

What is an enzyme?

A

Biological catalyst. Globular protein

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2
Q

Characteristics

A

-highly selective
-substrates into products
- proteins

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3
Q

The active site is

A

Where the substrate attaches

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4
Q

Binding site

A

Holds substrate in place

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5
Q

Catalytic site

A

Where reaction occurs

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6
Q

Specific sequence
●○○○○○●○○○○●

A

○○○● ●○○
○○●○○○○
○○○○

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7
Q

Lock and key model

A
  • active site has a rigid shape
  • only substrate with matching shape can fit
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8
Q

Induced fit model

A
  • flexible active site
  • can change shape to accommodate
  • bigger range of substrate specificity
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9
Q

Naming enzymes

A

End in ase after substrate they act on

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10
Q

Protease- uses

A

Pre digest protein during manufacturing baby foods

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11
Q

Lipase- use

A

Used with protease
Biological detergents to break down
Stains to smaller water soluble substances

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12
Q

Carbohydrase- use

A

Convert starch syrup, e.g, sports drinks

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13
Q

Isomerase- use

A

Glucose syrup to fructose

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14
Q

EC 1

A

Oxidoreductases

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15
Q

EC2

A

Transferase

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16
Q

EC 3

A

Hydrolases

17
Q

EC 4

A

Lyases

18
Q

EC 5

A

Isomerases

19
Q

EC 6

A

Ligases

20
Q

Naming system
(1.2.3.4)

A

1 is EC
2 is subclass
3 is sub sub class
4 is the serial number in its subclass

21
Q

Co enzyme

A

Non protein organic molecules

22
Q

Holoenzyme

A

Enzyme + Co enzyme

23
Q

Apoenzyme

A

Inactive enzyme alone

24
Q

Organic co enzymes
The Big Red Nose

A

Thiamine
Biotin
Riboflavin
Niacin

25
Q

Co factors

A
  • Inorganic species/ nonprotein
  • speeds up
  • not substrate of reaction
26
Q

Inorganic co factors examples

A
  • Mg
    -Fe
    -Zn
    -Mn
27
Q

Activation energy

A
  • bringing reactants together
  • formation of unstable bonds
  • bond rearrangements
28
Q

Effect enzyme activity

A
  • pH
  • temperature
  • salt concentration
  • substrate concentration
  • co enzymes/ Co factors
29
Q

Optimum temperature

A

37° - human body temp

30
Q

Inhibitors

A

Decrease enzyme activity

31
Q

Inactivators

A

Irreversible inhibitors

32
Q

Reversible inhibitors

A

Dissociate from the enzyme

33
Q

Types of inhibitors

A
  • competitive
  • non-competitive
  • uncompetitive