Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CC > enzymes > Flashcards

enzymes Flashcards

1
Q

enzyme in Bilirubin enzymatic reaction

A

Bilirubin oxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

highest elevation of CK >5xULN

A

Duchenne’s muscular dystrophy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

CK tissue sources

A

BHS
Brain, heart (cardiac ), Skeletal muscle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

CK Isoenzyme in severe tissue damage

A

CK-Mi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

major CK isoenzyme in healthy individuals

A

CK-MM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

CK Forward determination

A

Tanzer-Gilvarg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

CK Reverse determination

A

Oliver-Rosalki

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

CK: Ref value:
Male: 15-160U/L (37°C)
Female : 15-130 U/L (37°C)

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Tissue sources Of LDH

A

Heart, RBCS, Renal cortex, Lungs, Liver, Skeletal muscle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Normal pattern LDH

A

LD2>LD1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

LDH pattern assoc. w/ AMI, Renal Infarction Hemolysis

A

LD1>LD2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

highest elevation of LDH >5xULN

A

megaloblastic/pernicious anemia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Cold Labile LDH isoenzyme

A

LD5 (most) and LD4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

ck enzyme labile when stored

A

ck 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Forward method LDH determination

A

Wacker

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Reverse LDH determination

A

Wroblewski-Ladue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Reference value LD
100-225 U/L @ 37°C

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

ck starts to increase AMI
a 4-6 hrs
b. 6-8 hrs
C. 12-24hrs

A

4-6 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

AMI,ck peaks at
a.48-72hrs
b. 12-24 hrs
c.18-24hrs

A

12-24 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

AMI,ck normalize in

a. 10 days
b. 4-5 days
C. 2-3 days

A

2-3 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

AMI, AST starts to elevate

a. 4-6 hrs
b.6-8hrs
C. 12-24hrs

A

6-8 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

AMI, AST peaks at

a.48-72hrs
b. 18-24hrs
C. 12-24 hrs

A

18-24 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

AMI, AST normalize in

a. 10 days
b.2-3 days
C. 4-5 days

A

4-5 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

AMI, LD starts to increase

a. 6-8 hrs
b. 4-6 hrs
C.12-24 hrs

A

12-24 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

AMI, LDH peaks at

a . 18-24 hrs
b. 12-24hrs
C.48-74 hrs

A

48-74 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

AMI, LDH Normalize

a 4-5 days
b. 10 days
C.2-3 days

A

10 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

AST/SGOT tissue sources

A

Liver, heart, skeleletal muscle

28
Q

ALT/SGPT tissue sources

A

Liver

29
Q

ALT/SGOT >5XULN

A

Acute hepatocellular disorders

30
Q

De Ritis Ratio

A

AST/ALT <1

31
Q

no isoenzyme

A

ALT/SGPT

32
Q

AST/ALT Determination

A

Reitman-Frankel w/ COD enzymatic reaction

33
Q

AST: 5-35 U/L
ALT: 7-45U/L

A
34
Q

ALP tissue sources

A

liver, bone, placenta, intestine (Spleen, kidney)

35
Q

Highest ALP elevation >5ULN

A

paget’s disease/osteitis deformans

36
Q

Electrophoresis ALP is oenzyme anodic - cathodic

A

Liver
Bone
PIacenta
Intestine

37
Q

Heat stability in ALP isoenzymes most heat stable – heat labile

A

placenta, intestine, liver, bone

38
Q

urea inhibits

A

Bone

39
Q

phenylalanine inhibits

A

placental, intestinal, carcinoplacental isoenzyme

40
Q

Levamisole inhibits

A

Liver and bone

41
Q

Leucine inhibits

A

Nagao

42
Q

Intestinal depends on the blood group and secretor status more likere to be found in

A

B and O secretor individuals

43
Q

ALP ref. method

A

Bowers-McComb

44
Q

ALP Ref value
30-90 U/L

A
45
Q

Acute pancreatitis, AMS
occur : 2-12hrs
peak: 24hrs
normalize 3-4 days

A
46
Q

Acute pancreatitis, Lipase
occur : 4-8 hrs
peak: 24 hrs
Normalize : 8-14 days

A
47
Q

Ref. AMS determination

A

Saccharogenic Method

48
Q

ams determination

S accharogenic - I Inc. keducing sugar (Alcohol)
A myloclastic - decrease color intensity
C homogenic- Increas color intensity
C OD Enzymatic mtd-Increase ABSORBANCE 340 nm

A
49
Q

AMS NV
Serum: 25-130 U/L
Urine: 1-15 U/h (faster clearance due to small size)

A
50
Q

Salivary AMS inhibited by

A

Wheat germ Lectin

51
Q

Lipase determination

A

Cherry Crandall Method

52
Q

Cherry Crandall Method
uses: Olive Oil or Triolein as substrate
Reagent: TAG
measured: Titration

A
53
Q

lipase Ref. value
0-1.0 U/mL (<38 U/L)

A
54
Q

GGT increase

A

Alcoholic liver disease

55
Q

GGT REF.value
male: 55 U/L
Female : 38 U/L

A
56
Q

Increase 5 ‘Nucleotidase

A

Intrahepatic cholastasis

57
Q

Increase ALP, GGT, 5’nucleotidase

A

LIVER

58
Q

Increase ALP
N° GGT, 5’nucleotidase

A

BONE

59
Q

ACP tissue sources

A

HIGH: prostate glands

Low: Bone, placenta, erythrocyte, platelets

60
Q

ALP determination

A

Roy method

61
Q

Roy method uses

A

Thymolphthalein monophosphate

62
Q

Prostatic ACP is inhibited by

A

tartrate

63
Q

RBC ACP is ihibited by

A

Formaldehyde and copper

64
Q

Ref range ACP

total ACP:
male: 2.5-11.7U/L
Female : 0.3-9.2 U/L

prostatic ACP
male: 0.2-5.0 U/L
Female : 0.0-0.8U/L

A
65
Q

G6PD tissue sources

A

RBCs, adrenal glances, thymus, lymph nodes, spleen

66
Q

Drug Induced Gemolitic anemia def. in what enzyme

A

G6PD

67
Q

Antimalarial drugs/primaquine can cause des in what enzyme

A

G6PD - drug induced hemolytic anemia

CC (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions