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Biological Chemistry > Enzymes > Flashcards

Enzymes Flashcards

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1
Q

What is the function of beta galactosidase

A

to hydrolyse lactose (products are galactose and glucose) and other galactosides

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2
Q

Michaelis - menten equation

A

v = vmax ([s]/km+[s])

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3
Q

What is vmax

A

the maximal velocity

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4
Q

What is km

A

the substrate concentration that gives a velocity of half of vmax

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5
Q

Kcat formula

A

(K2+Kcat)/K1

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6
Q

What is the rate of formation proportional to

A

the concentration of the enzyme substrate complex

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7
Q

Rate of change[P] formula

A

Kcat[ES]

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8
Q

Vmax formula

A

Kcat x [Etot]

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9
Q

What is vmax directly proportional to

A

the enzyme concentration

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10
Q

Substituting M-M equation

A

v = kcatEtot

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11
Q

V=kcat[Etot] is dependent on what

A

has linear dependence on enzyme concentration

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12
Q

[S]/(Km+[S]) has dependence on what

A

hyperbolic dependence on substrate concentration

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13
Q

specifity constant

A

Kcat/Km

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14
Q

Features of a good enzyme

A

High Kcat, low Km, Kcat/km is high

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15
Q

What is a lineweaver-burke plot

A

1/v plotted against 1/[S]

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16
Q

What does the x and y intercept show on a lineweaver-burke plot

A

X = -1/km. Y = 1/Vmax

17
Q

Lineweaver-Burke plot equation

A

1/v = Km/vmax 1/[S] + 1/Vmax

18
Q

What is the enzyme transition state

A

highest energy state in a reaction, the binding of a substrate stabilise them and lowers the energy barrier

19
Q

What is reversible inhibition

A

competitive inhibition - competing to bind to the active site so the substrate can’t

20
Q

Velocity with inhibitor equation

A

V = Vmax ([s]/(km(1+([I]/Ki)) + [S])

21
Q

What is kmapp formula

A

km (1+([I]/Ki))

22
Q

What is Km

A

the real value for the interaction of the enzyme and substrate

23
Q

What is km apparent

A

what it seems to have chnaged during the experiment

24
Q

What is a good Ki value

A

low - less inhibitor needed for desired effect

25
Q

Inhibitors in diagnosis of diseases

A

Myasthaenia gravis is progressive muscular weakness. The problem is at neuromuscular junction, where the receptors are blocked so acetyl choline can not bind. Condition can be relieved by raising concentration of acetylcholine (increasing acetylcholinesterase)

Biological Chemistry (12 decks)

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