ENZYME (rodriguez)

Question 1

Proteins in nature produced by living cells that hasten chemical reaction in organic matter

Answer 1

Enzymes

Question 2

large molecules that are normally confined within cekls unless increased membrane permeability allows them to enter the blood

Answer 2

enzymes

Question 3

When does enxyme appear in serum

Answer 3

after cellular injury
degradation of cells
from storage areas

Question 4

these are crucial in the diagnosis and management of organ dysfunctions and diseases

Answer 4

tissue and cellular locations of enzymes

Question 5

factors affecting enzymatic reactions

Answer 5

Enzyme Concentration
Substrate Concentration
Cofactors
Isoenzymes
Temperature
Hydrogen Ion Concentration or pH
Storage
Hemolysis
Lactescense or milky specimen

Question 6

what happens to enzymatic reaction if there is high enzyme concentration

Answer 6

enzymatic reaction us faster because more enzyme is present to bind with the substrate

Question 7

(true or flase) with the amount of enzyme exceeding the amount of substrate, the reaction rate steadily decreases as more substrate is added

Answer 7

false
decreases-increases

Question 8

(true or false) when substrate reaches a maximal value,higher concentration of substrate no longer results in increased rate of reactions

Answer 8

true

Question 9

nonprotein entities that must bind to particular enzymes before a reaction occurs

Answer 9

cofactors

Question 10

Cofactors

Answer 10

coenzymes
activators
metalloenzymes

Question 11

organic cofactor(second substrate)

Answer 11

Coenzyme

Question 12

why is coenzyne essential

Answer 12

to achieve absolute enzymatic activity

Question 13

what happens to enzymatic reaction when the concentration of coenzyme increases

Answer 13

it will increase the velocity of an enzymatic reaction

Question 14

examples of coenzyme

Answer 14

Nicotinamide adenine dinucleotide (NAD)
Nicotinamide Adenune dinucleotide phosphate (NADP)

Question 15

these are organic ions which alter the spatial configuratio of the enzyme for proper substrate binding

Answer 15

activators

Question 16

example of activators

Answer 16

calcium
zinc
Chloride
magnesium
potassium

Question 17

inorganic ions attached to a molecule

Answer 17

Metalloenzymes

Question 18

example of metalloenzymes

Answer 18

catalase
cytochrome oxidase

Question 19

Enzymatic reactions may not progress if this interferes with the reaction

Answer 19

Inhibitors

Question 20

Examples of inhibitors

Answer 20

Competitive inhibitor
non-competitive inhibitor
uncompetitive inhibitor

Question 21

it physically binds to the active site of an enzyme

Answer 21

Competitive Inhibitor

Question 22

(true or false) in competitive inhibitor, both the substrateand inhibitor compete for the same active site of the enzyme

Answer 22

true

Question 23

competitive inhibitor type of inhibition

Answer 23

reversible
substrates concentration is significantly higher than the concentration of inhibitor

Question 24

the effect of competitive inhibitor can be counteracted by adding

Answer 24

excess substrate to bind with the enzyme

Question 25

this results in reduction in the concentration of competitive inhibitor , thus increasing the rate of reaction

Answer 25

dilution of serum

Question 26

competitive inhibitor has the ability to alter

Answer 26

michaelis-menten constant (Km)

Question 27

it does not compete with the substrate but look for areas other than the active site

Answer 27

non-competitive inhibitor