Enzyme Lab

Question 1

Purpose

Answer 1

To compare the action of catalase to a non-protein catalyst under different conditions

Question 2

Materials (5)

Answer 2

3% hydrogen peroxide
manganese dioxide 
fresh or frozen liver
potato
ice

Question 3

Equipment (6)

Answer 3

fine clean sand
stirring rod
bunsen burner or hot plate
250 mL beaker
test tubes
scalpel

Question 4

How many test tubes are needed? (max-min)

Answer 4

4-11 test tubes

Question 5

Procedure (Headings) (A-E)

Answer 5

A: Catalytic Reactions
B: The effect on an Enzyme
C: Re-using an enzyme
D: Effect of particle size
E: Effect of temperature

Question 6

Procedure: A: Catalytic Reactions

Answer 6

Add 2mL hydrogen peroxide to 2 test tubes
Place 0.1g of sand in one test tube
Add 0.1g of manganese dioxide in the second test tube
Observe & Record reaction rates

Question 7

Procedure: B: The effect of an enzyme

Answer 7

Add 2mL of hydrogen peroxide to 2 clean test tubes
Place a small piece of liver in one test tube
Place a small piece of potato in the other test tube
Observe, Record and Compare (w/ A)

Question 8

Procedure C: Re-using an enzyme

Answer 8

Divide liquid portion of the previous tube in B w/ liver into 2 test tubes
Cut the liver from B into 2 equal parts & add to the 2 test tubes
Add another piece of liver to one of the test tubes
Add 1mL of hydrogen peroxide to other test tube
Observe & Record

Question 9

Procedure D: Effect of particle size

Answer 9

Place a small piece of liver in one test tube
Place a small piece of potato in another test tube
Add a pinch of sand to each tube
Crush these materials (out of the test tubes) with a stirring rod
Add 2mL of hydrogen peroxide to each test tube
Observe, Record and Compare with part B

Question 10

Procedure E: Effect of Temperature

Answer 10

Place a small piece of liver in a test tube & heat for 5 min in boiling water bath
Add 2mL hydrogen peroxide to the boiled liver
Record results

Place a small piece of liver into each of two test tubes
Place one test tube in 37 degree water for 5 min
Place one test tube in an ice water bath fro 5 min
Remove both test tubes from water
Add 2mL hydrogen peroxide into both tubes
Record the rates of reaction

Question 11

Chemical equation: A: Catalytic reactions

Answer 11

2mL H202 + 0.1g sand

2mL H202 + 0.1g MnO2

Question 12

Chemical equation: B: The effect of an enzyme

Answer 12

2mL H202 + liver

2mL H2O2 + potato

Question 13

Chemical equation: C: Re-using an enzyme

Answer 13

liver (1/2 from B) + liver (fresh)

liver (1/2 from B) + 1mL H2O2

Question 14

Chemical equation: D: Effect of particle size

Answer 14

liver + sand + 2mL H2O2

potato + sand + 2mL H2O2

Question 15

Chemical equation: E: Effect of temperature

Answer 15

liver (heated) + 2mL H2O2
liver (core temp) + 2mL H2O2
liver (chilled) + 2mL H2O2